Date (Creation)

2026-01-09

Date (Revision)

2026-01-09

Date (Publication)

2026-01-09

Date (released)

2026-01-09

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/0298edcf-93b1-4a6c-9a81-d9c785149b39

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/02135

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/S007431/1

Codespace

award

Other citation details

Please cite this item as: Sakovich, A., McClymont, E.L., Rowlands, E., & Manno, C. (2026). Lipid mass and free fatty-acid composition of Calanus hyperboreus (CV) exposed to pristine and biofouled microplastics during shipboard incubation experiments (southeastern Greenland, summer 2024) (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/0298edcf-93b1-4a6c-9a81-d9c785149b39

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Sakovich, Alena		Author
Durham University	McClymont, Erin L.		Author
British Antarctic Survey	Rowlands, Emily		Author
British Antarctic Survey	Manno, Clara		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• Arctic copepods

• Greenland

• lipid dynamics

• plastic pollution

Place

• Southeast Greenland Arctic

GEMET - INSPIRE themes, version 1.0

• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

public access limited according to Article 13(1)(e) of the INSPIRE Directive

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data are supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

Data are under embargo until publication of the associated manuscript.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2024-07-25

End date

2024-08-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Sampling and experiments were conducted between 2024-07-25 and 2024-08-31. Sampling of copepods for Experiment I was performed on 2024-07-24, for Experiment II - on 2024-08-04 (first morning hours), and for Experiment III - on 2024-08-17 and 2024-08-18. The duration of Experiment I was from 2024-07-27 to 2024-08-02, Experiment II - from 2024-08-04 to 2024-08-10, and Experiment III - from 2024-08-19 to 2024-08-25.

Copepods were collected along the southeastern Greenland shelf with a motion-compensated 200 µm Bongo net. Copepods for Experiment I and Experiment II were collected from individual single stations (67.492° N, 33.003° W and 68.107° N, 30.423° W, respectively). For Experiment III, copepods were collected from three stations: two adjacent sites (67.414° N, 32.683° W; 67.411° N, 32.683° W) and a third site about 31 km to the north (67.693° N, 32.577° W).

Immediately after capture, copepods were acclimated for 12-24 h in 1 L beakers with 1.2 µm-filtered Arctic seawater at 7 °C. Only active, undamaged individuals were used. Three shipboard experiments were conducted: Experiment I exposed copepods to pristine microplastics (MP) with food, Experiment II to pristine MP without food, and Experiment III to biofouled MP with food. Each experiment included Control (0 MP), Low MP (20 µg L-1), and High MP (200 µg L-1) treatments. Experiments I and II used three replicate 50 mL Falcon tubes with two copepods per tube. Experiment III used three replicate 50 mL Falcon tubes with one copepod per tube due to limited specimen availability at that time of the season. Fed treatments received a mixed algal diet (Nannochloropsis, Tetraselmis, Pavlova, Isochrysis, Thalassiosira weissflogii; Reefphyto, UK) at 4,600 cells mL-1 every two days. Tubes were mounted horizontally on a vertical rotator (1 rpm, 90° tilt) and opened every two days for feeding (where applicable), and gentle aeration.

Microplastics consisted of polyamide nylon-6 powder (Goodfellow, AM306010). Pristine MP were prepared in a clean, plastic-free laboratory at British Antarctic Survey before cruise commencement via size-fractionation to 5-25 µm, and dilution (0.25 mg in 25 mL 2 µm-filtered Milli-Q water) to produce a 0.01 mg mL-1 stock suspension. Defined volumes of this stock (100 µL and 1 mL) were then pipetted before experiment commencement into 50 mL Falcon tubes and diluted to a full volume with 1.2 µm-filtered Arctic seawater to obtain final MP concentrations of 20 and 200 µg L-1. For biofouled MP, 1 mg of particles was prepared identically, transported on board in pre-cleaned aluminium foil envelope, and incubated in 100 mL Arctic seawater for 27 days at 7 °C with constant mixing. Immediately before the experiments, defined volumes of the continuously mixed suspension were pipetted directly into Falcon tubes and brought to 50 mL to achieve equivalent MP concentrations.

At the end of each experiment, no mortality or behavioural changes were observed. Copepods were rinsed and frozen individually at -80 °C.

At the stage of the lipid extraction, copepods were freeze-dried for 24 h, and two or three individuals from different tubes were pooled per replicate to account for inter-individual variability and to meet FA detection limits (n = 3 per treatment). Copepods were then weighed for dry mass, and spiked with 20 µL of 5alpha(H)-cholestane as the internal standard. Lipids were extracted using dichloromethane:methanol (9:1 v/v), with sonication and 24 h incubation with solvent, evaporated under vacuum at 30 °C, re-dissolved, and dried under nitrogen gas flow before gravimetric quantification (µg lipid mg-1 DW ind-1). Lipids were then methylated with methanol containing 5% HCl at 70 °C for 12 h, extracted with hexane:dichloromethane, dried, and silylated wi...(16)

Data collection:

gas chromatography-mass spectrometry (GC-MS)

LIPID MAPS spectral library

R version 4.3.2

Data quality:

For MP preparation and experimental work, all materials were pre-cleaned three times with Milli-Q water and once with ethanol (2 µm-filtered). All laboratory procedures for lipid extraction were carried out using pre-combusted or solvent-rinsed glassware to minimise contamination. Procedural blanks were included alongside all samples throughout lipid extraction, silylation, and GC-MS analysis to quantify background contamination. Fatty-acid peaks detected in blanks at corresponding retention times were removed from sample chromatograms before further analysis. Biological replication was maintained at the incubation level during experiments. Individuals were then pooled (2-3 specimens) within replicates to ensure sufficient material for lipid extraction and to reduce individual variability, yielding a final replication of n=3 per treatment for each experiment, including the control treatment without MP under identical conditions. No mortality was observed during incubations, and no samples were excluded based on behavioural criteria. Lipid mass was normalised to dry weight to account for size differences among individuals. Fatty-acid composition is reported as relative abundance (% of total detected fatty acids). Only fatty acids contributing more than 1% of total fatty-acid abundance across all samples were retained to reduce analytical noise. No missing values are present, and no data interpolation or imputation was applied.

Metadata

File identifier

0298edcf-93b1-4a6c-9a81-d9c785149b39 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2026-01-09

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact