BGS Hosted Metadata
Images of histological sections of oocytes for the bivalves Astarte crenata and Bathyarca glacialis from the Western Barents Sea under ambient and near-future climate change conditions.
Images of histological sections of oocytes to quantify the interactive effects of ocean warming and ocean acidification based on near-future climate change projections on oocyte size frequency distributions of benthic invertebrates (the bivalves Astarte crenata and Bathyarca glacialis) from the Western Barents Sea.
Supported by The Changing Arctic Ocean Seafloor (ChAOS) - how changing sea ice conditions impact biological communities, biogeochemical processes and ecosystems project (NE/N015894/1 and NE/P006426/1, 2017-2021), Natural Environment Research Council (NERC) in the UK.
Simple
- Date (Creation)
- 2021-09-17
- Date (Revision)
- 2021-09-17
- Date (Publication)
- 2021-09-17
- Date (released)
- 2021-09-17
- Edition
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1.0
- Unique resource identifier
- https://doi.org/10.5285/04766a75-502c-4431-b771-107326a8585c
- Codespace
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doi
- Unique resource identifier
- GB/NERC/BAS/PDC/01564
- Codespace
- Unique resource identifier
- NE/N015894/1
- Codespace
-
award
- Unique resource identifier
- NE/P006426/1
- Codespace
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award
- Other citation details
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Please cite this item as: Reed, A., Godbold, J., Solan, M., & Grange, L. (2021). Images of histological sections of oocytes for the bivalves Astarte crenata and Bathyarca glacialis from the Western Barents Sea under ambient and near-future climate change conditions. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/04766a75-502c-4431-b771-107326a8585c
- Credit
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No credit.
- Status
- Completed
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Southampton
Reed, Adam Author University of Southampton
Godbold, Jasmin Author University of Southampton
Solan, Martin Author Bangor University
Grange, Laura Author NERC EDS UK Polar Data Centre Point of contact
- Maintenance and update frequency
- As needed
- Maintenance note
- Completed
- Theme
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dynamic energy budget
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functional response
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life history
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metabolic plasticity
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oogenesis
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- Place
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Western Barents Sea Arctic
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GEMET - INSPIRE themes, version 1.0
- Access constraints
- Other restrictions
- Other constraints
- no limitations to public access
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- License
- Other constraints
- Open Government Licence v3.0
- Use constraints
- Other restrictions
- Other constraints
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This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/
- Use constraints
- Other restrictions
- Other constraints
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This data is governed by the NERC data policy and supplied under Open Government Licence v.3
- Unique resource identifier
- url
- Codespace
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url
- Association Type
- Larger work citation
- Unique resource identifier
- url
- Codespace
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url
- Association Type
- Cross reference
- Unique resource identifier
- url
- Codespace
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url
- Association Type
- Cross reference
- Unique resource identifier
- url
- Codespace
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url
- Association Type
- Cross reference
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
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- Begin date
- 2017-07-22
- End date
- 2017-08-01
- Supplemental Information
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It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.
- Title
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European Petroleum Survey Group (EPSG) Geodetic Parameter Registry
- Date (Publication)
- 2008-11-12
- Cited responsible party
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Organisation name Individual name Electronic mail address Role European Petroleum Survey Group
Publisher
- Unique resource identifier
- urn:ogc:def:crs:EPSG::3031
- Version
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6.18.3
Distributor
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Distributor
- Distributor format
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Name Version image/jpeg
- Units of distribution
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bytes
- Transfer size
- 7902739825
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=04766a75-502c-4431-b771-107326a8585c Get Data
- Hierarchy level
- Dataset
- Statement
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Methodology:
Specimens were collected from each of 1 station in 2017 (B13) and 2 stations in 2018 (B16 and B17) during two consecutive cruises (RRS James Clark Ross: JR16006, 30th June to 8th August 2017; JR17007: 10th July to 5th August, 2018) following a transect along the 30°E meridian. At each station similar-sized specimens of the infaunal bivalves Astarte crenata and Bathyarca glacialis were obtained by Agassiz trawl. Surficial sediment < 10 cm deep was collected using SMBA box cores, sieved to remove macrofauna (500 µm mesh), and homogenised by stirring. Both infaunal bivalves and sediment were maintained under aerated seawater and returned to the Biodiversity and Ecosystem Futures Facility, University of Southampton at ambient temperature (1.5 + 1°C). Ten centimetres of homogenised sediment were transferred to transparent acrylic aquaria (internal dimensions, LWH: 20 x 20 x 34 cm) and overlain with ~8 L (20cm depth) surface seawater (salinity, ~34). Aquaria were maintained in the dark at ambient conditions (1-2 °C and ~400 ppm [CO2]), and randomly distributed between two insulated fibreglass seawater baths (LWH: 1.2 x 1.2 x 0.8m, Tanks Direct, UK). Three B. glacialis were introduced to each of twelve aquaria (n = 36; ambient 18, future 18) and six A.crenata were introduced to each of ten aquaria (n = 60; 30 ambient, 30 future). After a 30-day acclimation period to aquarium conditions, temperature and [CO2] were manually adjusted (1 °C and ~100 ppm CO2 week -1) to achieve near-future treatment conditions (3-5 °C and ~550 ppm [CO2]). We periodically measured pH, temperature and salinity and total alkalinity. The bivalves were fed ad libitum three time per week (100 ml cultured live Isochrysis sp., Tetraselmis sp., and Phaeodactylum sp.), and overlying water exchanged weekly. Experiments were run for 120 days (B. glacialis, 21//11/2017-20/03/2018) or 135 days (A. crenata, 08/10/2018-19/02/2019) after which animals were removed and fixed in 4 neutral buffered formaldehyde.
Oocyte images: Images of stained slides of histological sections from individual females were captured using a Nikon D5000 digital SLR camera mounted on an Olympus (BH-2) stereomicroscope and analysed using ImageJ v 1.48. Slides were photographed at X10 magnification. Replicate photographs were taken to ensure 100 unique oocytes were measured for each female. Composite images were generated for easy analysis wherever possible. Full descriptions and additional information provided in Reed et al. 2021 Frontiers in Marine Science in main manuscript and supplementary information.
Data collection:
Resolution:
Biometric measurements (i.e., shell length, shell height and tumidity (width) to two decimal places.
Wet weight to four decimal places.
Oocyte Equivalent Circular Diameter to two decimal places.
Instrumentation:
Mettler-Toledo InLab Expert Pro temperature-pH combination electrode
Mettler-Toledo InLab 737 IP67 temperature-conductivity combination electrode
Digital vernier calliper
Balance (+/-0.000g)
Rotary microtome
Nikon D5000 digital SLR camera
Olympus BHS (BH-2) stereomicroscope
Data quality:
Standard protocols were followed and data entry double checked by independent person.
Metadata
- File identifier
- 04766a75-502c-4431-b771-107326a8585c XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2021-09-17
- Metadata standard name
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ISO 19115 Geographic Information - Metadata
- Metadata standard version
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ISO 19115:2003(E)
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Point of contact
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