06ea87f3-476d-40fd-acce-e6923e786d48 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:23:58 UK GEMINI 2.3 OSGB 1936 / British National Grid Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2 2022-03-08 publication https://catalogue.ceh.ac.uk/id/06ea87f3-476d-40fd-acce-e6923e786d48 10.5285/06ea87f3-476d-40fd-acce-e6923e786d48 doi: Leitão, A.B., Ramesh, A., Day, J.P., Hanna, N., Devi, A., Hayes, M.P., Jiggins, F.M. (2022). Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2. NERC EDS Environmental Information Data Centre 10.5285/06ea87f3-476d-40fd-acce-e6923e786d48 Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics. The data were produced under the grant: NE/P00184X/1 Why does Drosophila vary in susceptibility to parasitoid wasps. Full details about this dataset can be found at https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48 Leitão, A.B. University of Cambridge ac2016@cam.ac.uk https://orcid.org/0000-0001-9004-1831 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Ramesh, A. University of Cambridge ar885@cam.ac.uk https://orcid.org/0000-0003-0050-3516 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Day, J.P. University of Cambridge jd642@cam.ac.uk author Hanna, N. University of Cambridge naeeh2@cam.ac.uk author Devi, A. University of Cambridge aarathidevi@hotmail.com author Hayes, M.P. University of Cambridge mph51@cam.ac.uk author Jiggins, F.M. University of Cambridge fmj1001@cam.ac.uk https://orcid.org/0000-0001-7470-8157 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Ramesh, A. University of Cambridge ar885@cam.ac.uk https://orcid.org/0000-0003-0050-3516 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Cambridge ar885@cam.ac.uk owner Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Drosophila melanogaster Leptopilina boulardi Wikidata 2012-10-29 creation genetics encapsulation parasite GEMET - Concepts, version 4.1.3 2019-01-28 creation RNA-sequencing parasitoid wasp differential gene expression fat body haemocyte Evolutionary genetics insect genomics otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Leitão, A.B., Ramesh, A., Day, J.P., Hanna, N., Devi, A., Hayes, M.P., Jiggins, F.M. (2022). Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48 textTable English utf8 biota 2017-10-01 2017-10-31 -1.464 1.768 51.232 52.974 Comma-separated values (CSV) txt NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/06ea87f3-476d-40fd-acce-e6923e786d48 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/06ea87f3-476d-40fd-acce-e6923e786d48.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied, and functional enrichment was done.