12f2f235-8e18-40c7-9113-acb85de9edd7 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-03-04T09:03:49 UK GEMINI 2.3 Phenotypic traits of Rhizobium leguminosarum TRX19 clones evolved in the presence of different lytic bacteriophages 2026-03-04 publication 2025-09-04 creation https://catalogue.ceh.ac.uk/id/12f2f235-8e18-40c7-9113-acb85de9edd7 This dataset comprises phenotypic data of Rhizobium leguminosarum TRX19 clones evolved in the presence or absence of lytic phages. The clones assayed were isolated from populations of R. leguminosarum which had been evolved in lab media for twenty 73hr 'transfers'. Clones were assessed for their resistance to the ancestral phages (reduction in bacterial growth), their ability to form symbioses (plant biomass, nitrogen content and nodule counts) and traits associated with colonisation (biofilm production, swimming and swarming motility and gelatinase activity). This experiment tests the hypothesis that evolution for phage resistance will drive the loss of symbiosis functions in rhizobia due to antagonistic pleiotropy. Ellie Harrison University of Sheffield ellie.harrison@sheffield.ac.uk pointOfContact Pain, C. University of Sheffield capain1@sheffield.ac.uk author Harrison, E. University of Sheffield ellie.harrison@sheffield.ac.uk https://orcid.org/0000-0002-2050-4631 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Sheffield EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher symbiosis GEMET - Concepts, version 4.1.3 2019-01-28 creation Evolutionary ecology Bacteriophage Rhizobia Antagonistic coevolution otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence English utf8 environment Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 This dataset contains assay data from an evolution experiment assessing the impact of evolution with different lytic phages on the symbiotic performance of rhizobia. Briefly, strain Rhizobium leguminosarum TRX19 was evolved in lab media with 1 of 3 phages or in the absence of phage. Clones were isolated from evolved populations and screened for resistance, symbiotic function and phenotypes related to colonisation. Most of the data are raw values acquired from assays of the evolved clones. These include: growth with host plant - collecting plant biomass, nodule counts and nitrogen content via Isotope ratio mass spectrometry. Phenotypic analysis - biofilm production, swimming and swarming motility, growth rate in liquid broth and gelatinase assays Phage resistance data is presented as RBG - reduction in bacterial growth - generated as the ratio of the density (OD600) of cultures grown in a microtitre plate either with or without phages. The ratio of phage exposed to phage free bacterial growth indicates the severity of the infection.