13f878dc-ef87-45d5-be8c-5733c88c30e9 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:16:55 UK GEMINI 2.3 WGS 84 Phenotypic and environmental data for Lake Ring, Denmark, Daphnia magna population, 1955-2010 2022-02-03 publication https://catalogue.ceh.ac.uk/id/13f878dc-ef87-45d5-be8c-5733c88c30e9 10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9 doi: Cambronero, M.C., Williams, T.D., Orsini, L., Colbourne, J.K. (2022). Phenotypic and environmental data for Lake Ring, Denmark, Daphnia magna population, 1955-2010. NERC EDS Environmental Information Data Centre 10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9 This dataset contains fitness-linked life history traits, environmental data, and protein and gene expression data for Daphnia magna. Life history data were measured in common garden experiments. For each clone of the same genotype across all experiments, size at maturity (distance between the head and the base of the tail spine), age at maturity (first time eggs were observed in the brood chamber), fecundity (total number of offspring released summing first and second brood), and mortality were measured. Critical thermal maximum (CTmax) was measured on the experimental animals. Environmental data consisted of Secchi disk depth (water transparency) ; total phosphorous; and total nitrogen for the years 1971–1999; a record of pesticides 1955–2010; temperature records over the past century 80 km from Lake Ring. Organic and carbonate contents of the sediment was estimated using the loss on ignition (LOI) method. Hb protein data consisted of constitutive Hb protein crude content animals reared in normoxic (saturated oxygen level) conditions at two experimental temperatures, 20 and 30°C. Heat shock protein expression was measured in four heat shock proteins (HSP20, HSP60, HSP70 and HSP90). Total RNA, qPCR, mean CT (cycle threshold) value per sample and per protein were collected. Full details about this dataset can be found at https://doi.org/10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9 Cambronero, M.C. University of Birmingham mc.cuenca.maria@gmail.com https://orcid.org/0000-0002-5342-2165 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Williams, T.D. University of Birmingham T.D.WILLIAMS@bham.ac.uk https://orcid.org/0000-0002-5857-3851 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Orsini, L. University of Birmingham l.orsini@bham.ac.uk https://orcid.org/0000-0002-1716-5624 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Colbourne, J.K. University of Birmingham J.K.Colbourne@bham.ac.uk https://orcid.org/0000-0002-6966-2972 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Colbourne, J.K. University of Birmingham J.K.Colbourne@bham.ac.uk pointOfContact University of Birmingham EMAIL NOT PROVIDED owner Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Daphnia magna Wikidata 2012-10-29 creation pesticide eutrophication GEMET - Concepts, version 4.1.3 2019-01-28 creation Resurrection ecology Freshwater ecology Experimental evolution Fitness traits otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Cambronero, M.C., Williams, T.D., Orsini, L., Colbourne, J.K. (2022). Phenotypic and environmental data for Lake Ring, Denmark, Daphnia magna population, 1955-2010. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9 textTable 15 English utf8 biota 1955-01-01 2010-01-01 9.588 9.605 55.961 55.968 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/13f878dc-ef87-45d5-be8c-5733c88c30e9 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/13f878dc-ef87-45d5-be8c-5733c88c30e9.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Lineages (genotypes) of Daphnia were resurrected (revived) from biological archives of Lake Ring, a shallow mixed lake in Denmark (550 57´ 51.83´´ N, 9035´46.87´´E) with a known history of anthropogenic impact. The process by which lineages were obtained is as follows: 1. Sediment is sampled using a piston corer of approximately 1.5 m in length with an internal tube diameter of 14 cm 2. The sediment core is separated in incremental layers of 1 cm or less using a flat metal surface 3. Each sediment layer is collected in a separate sampling bag and weighted using a precision scale 4. Each layer of sediment is sieved and dormant Daphnia isolated after visual inspection 5. Under a stereo microscope, each dormant Daphnia encapsulated in an ephippium is opened using microdissection forceps to expose dormant embryos to light 6. Each dormant embryos develops in a fully functional adult from which an isoclonal lineage is established and maintained in the laboratory indefinitely. From a total of 923 dormant Daphnia, 262 lineages were successfully hatched. From these, a total of 40 randomly chosen lineages across the sediment core were used in the experiments and fitness-linked life-history traits measured.