16649ff0-af24-41b0-bcb4-15e610dac170 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:23:07 UK GEMINI 2.3 OSGB 1936 / British National Grid Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments 2014-05-14 publication 2010-01-01 creation 1395928899368 CEH:EIDC: https://catalogue.ceh.ac.uk/id/16649ff0-af24-41b0-bcb4-15e610dac170 10.5285/16649ff0-af24-41b0-bcb4-15e610dac170 doi: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre 10.5285/16649ff0-af24-41b0-bcb4-15e610dac170 This dataset contains operational taxonomic units for epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample. Full details about this dataset can be found at https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170 Griffiths, R. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact Griffiths, R. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Edwards, F. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Scarlett, P. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Webb, G. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Sarlej, R. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Thomson, B. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner unknown not planned Land Use theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication microbiology genetics GEMET - Concepts, version 4.1.3 2019-01-28 creation Biodiversity genetic information Tellus South West project otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions If you reuse this data, you should cite: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon bacteria in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/16649ff0-af24-41b0-bcb4-15e610dac170 textTable English utf8 biota environment inlandWaters location 2013-10-01 2014-03-20 -6.452 1.768 49.864 55.812 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/16649ff0-af24-41b0-bcb4-15e610dac170 Download the data Get a copy of this data download https://data-package.ceh.ac.uk/sd/16649ff0-af24-41b0-bcb4-15e610dac170.zip Supporting Information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.