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Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm

In this mesocosm study, three types of wet wipes (plastic containing, and home and commercially compostable) colonised with E. coli were buried in beach sand and their degradation, tensile strength, and concentration of E. coli was quantified over 15 weeks. Wipes were firstly passed through a series of treatments to simulate their journey from the bathroom to the beach. Water characteristics (pH, salinity) of these water types were measured. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d

Simple

Date (Publication)
2024-07-15
Citation identifier
https://catalogue.ceh.ac.uk/id/19a8083b-afa4-40b9-91bf-7a529eea870d
Citation identifier
doi: / 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
Other citation details

Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d

Point of contact
Organisation name Individual name Electronic mail address Role
University of Stirling Metcalf, R.

Rebecca.metcalf@stir.ac.uk

Point of contact
University of Stirling Metcalf, R.

Rebecca.metcalf@stir.ac.uk

Author
University of Stirling

Fellows, R.

Rosie.fellows@stir.ac.uk

Author
University of Stirling

White, H.L.

Hannah.white@stir.ac.uk

Author
University of Stirling Quilliam, R.

Richard.quilliam@stir.ac.uk

Author
University of Stirling

Rebecca.metcalf@stir.ac.uk

Owner
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Publisher
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Custodian

GEMET - INSPIRE themes, version 1.0

  • Human Health and Safety

GEMET - Concepts, version 4.1.3

  • public health
  • microbiology
Keywords
  • Pollution
  • environmental pathogens

  • sewage discharge

  • plastic pollution

  • sand microbiology

Access constraints
Other restrictions
Other constraints
no limitations
Use constraints
Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
Other restrictions
Other constraints

If you reuse this data, you should cite: Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d

Language
English
Character set
UTF8
Topic category
  • Health
  • Environment
Distribution format
Name Version

Comma-separated values (CSV)

Distributor contact
Organisation name Individual name Electronic mail address Role

NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Distributor
OnLine resource
Protocol Linkage Name
https://data-package.ceh.ac.uk/data/19a8083b-afa4-40b9-91bf-7a529eea870d

Download the data

OnLine resource
Protocol Linkage Name
https://data-package.ceh.ac.uk/sd/19a8083b-afa4-40b9-91bf-7a529eea870d.zip

Supporting information

Hierarchy level
Non geographic dataset
Other

nonGeographicDataset

Conformance result

Title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication)
2010-12-08
Statement

At weekly timepoints (for 15 weeks), four of each wet wipe type were randomly selected, and removed from the mesocosm using sterile forceps. The loosely adhering sand was shaken off and each wet wipe transferred into a sterile glass vial containing 20 ml phosphate-buffered saline (PBS). Vials were then vortexed at 1500 rpm for 5 min and E. coli enumerated by filtering the PBS through 0.45 μm filters, which were placed onto selective media and incubated at 37°C. The wet wipes were removed from the glass vials and left to dry overnight. The following day, a 50 N digital force meter was used to quantify tensile strength by measuring the force required to break each wipe. Wipes were also photographed to determine degradation visually. Background bacterial concentrations were measured by filtering 100 ml water through 0.45 μm cellulose acetate membranes and placed onto selective media.

Metadata

File identifier
19a8083b-afa4-40b9-91bf-7a529eea870d XML
Metadata language
English
Character set
8859 Part 1
Hierarchy level
Non geographic dataset
Hierarchy level name

nonGeographicDataset

Date stamp
2025-11-13T16:15:02
Metadata standard name
UK GEMINI
Metadata standard version

2.3

Metadata author
Organisation name Individual name Electronic mail address Role
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Point of contact
 
 

Overviews

Keywords

GEMET - INSPIRE themes, version 1.0

Human Health and Safety


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