1cf37889-9f77-41b9-8c49-b175fbd03406 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2020-05-27 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Pseudogymnoascus roseus in soil at Mars Oasis, Alexander Island, under different temperature, irrigation and substrate treatments 2020-05-27 creation 2020-05-27 revision 2020-05-27 publication 2020-05-27 released 1.0 https://doi.org/10.5285/1cf37889-9f77-41b9-8c49-b175fbd03406 doi GB/NERC/BAS/PDC/01333 https://data.bas.ac.uk/ Please cite this item as: Newsham, K., Misiak, M., & Worland, M. (2020). Pseudogymnoascus roseus in soil at Mars Oasis, Alexander Island, under different temperature, irrigation and substrate treatments (Version 1.0) [Data set]. UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation. https://doi.org/10.5285/1cf37889-9f77-41b9-8c49-b175fbd03406 This dataset consists of field measurements of Pseudogymnoascus roseus DNA concentrations in soil and three edaphic factors (soil temperature, water potential and snow depth) at Mars Oasis, Alexander Island, from 2009 to 2012, under different temperature, irrigation and substrate (glucose, glycine and tryptone soy broth) treatments. Laboratory measurements of hyphal extension rates, conidial germination, numbers of conidia produced per colony of fungus and specific enzyme activities of three P. roseus isolates are also included. No credit. completed Newsham, Kevin K. British Antarctic Survey Researcher author Misiak, Marta British Antarctic Survey Researcher author Worland, M. Roger British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Fungi theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Mars Oasis Pseudogymnoascus roseus enzyme activity quantitative-PCR snow depth soil decomposer fungus soil warming soil water potential surface soil temperature theme Alexander Island, Mars Oasis Antarctica place Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions We strongly encourage contacting Kevin Newsham (kne@bas.ac.uk) for more information regarding this dataset, including guidance on its reuse. url url crossReference textTable eng utf8 biota -68.25 -68.25 -71.87833 -71.87833 2007-12-01 2018-12-31 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/csv text/plain bytes 4750049 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=1cf37889-9f77-41b9-8c49-b175fbd03406 WWW:LINK-1.0-http--link Get Data Download data download bytes 4750049 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=1cf37889-9f77-41b9-8c49-b175fbd03406 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: The field experiment was set up in December 2007, at Mars Oasis. 64 circular plots (1 m diameter) were established, half of which were covered with open top chambers. In December 2007, 2009, 2010, 2011 and 2012, each of the 64 plots was assigned to one of four groups (16 plots in each group) and the soil underwent four different treatments: unamended; received glucose; received glycine (amino acid); received powdered tryptic soy broth (mix of amino acids and protein). Half of the chambered and half of the unchambered plots were then raised to 100% water holding capacity. The experimental layout resulted in 16 open top chamber-irrigation-substrate treatment combinations, each replicated four times in a randomised design. Temperature was monitored in three chambered and three unchambered plots between December 2009 and December 2012. Frozen soil samples were returned to the UK, where water potentials were measured. Laboratory experiments were also undertaken. Three Pseudogymnoascus roseus isolates were obtained from Mars Oasis soil using the soil plate method and identified by sequencing of ribosomal DNA. Quantitative-PCR assays were used to estimate the concentration of Pseudogymnoascus DNA present in soil. The hyphal extension rate analyses were undertaken in the lab, where isolates were grown for 10 weeks on soil extract medium at temperatures cycling daily from 2-18 ° C, 2-21 ° C and 2-24 ° C and at water potentials of -1.06 MPa, -3.60 MPa, -6.00 MPa and -10.80 MPa. At the end of the growth rate experiments, extracellular enzymes were extracted from colonies and the activities of β -1,4-glucosidase (cellulase), N-acetyl- β -glucosaminidase (chitinase), leucine aminopeptidase and alkaline and acid phosphatases were measured using p-nitrophenyl substrates. The impact of temperature cycle and water potential treatments on numbers of P. roseus conidia produced per colony was measured. The treatment effects on conidial germination were also quantified at modified water potentials. Complete methods can be downloaded with the data via the Get Data link. Data collection: Soil temperatures: TinyTag Plus 2 TGP-4017, Gemini Data Loggers Ltd., Chichester, UK Soil water potentials: WP4C, Decagon Devices, Inc., Pullman, WA, USA Q-PCR thermocycler: Eco 48 machine, PCRMax Ltd., Witford, UK Image analysis: ImageJ, National Institute of Health, Bethesda, MD, USA Plant growth cabinets: MCR-350 Versatile Environmental Test Chamber, Sanyo, Osaka, Japan Plate reader: Sunrise, TECAN, Austria Data quality: Raw data are shown. The data have not been cleaned. Missing data are indicated by NA. There are 1-2 missing data points in the water potential, DNA concentration, conidial density and hyphal extension rate data, caused principally by lack of material or analysis failure. 22 values are missing from the specific enzyme activity data, all of which were caused by analysis failure. asNeeded completed