1e435dd3-ed93-4961-a136-ffa477731e1c English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:21:15 UK GEMINI 2.3 WGS 84 Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016) 2017-04-21 publication 2016-04-19 creation https://catalogue.ceh.ac.uk/id/1e435dd3-ed93-4961-a136-ffa477731e1c 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c doi: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c The data set includes the results of a laboratory analysis in 2016, investigating the presence of trypanosomes and prevalence of tsetse endosymbionts in tsetse flies. The tsetse flies were sampled in Hurungwe District, Mashonaland West Province, Zimbabwe, from February 2014 to November 2014. Flies were sampled using a combination of Epsilon traps and fly rounds, both established techniques for sampling tsetse. Tsetse were stored prior to laboratory analysis using Polymerase chain reaction (PCR) techniques in 2016. The data include two species of tsetse, Glossina pallidipes and Glossina morsitans morsitans. Trypanosome species investigated include Trypanosoma brucei s.l., T. b. rhodesiense, T. vivax, T. congolense, T. simiae, T.simiae (Tsavo) and T. godfreyi. Endosymbionts included in the study were Sodalis glossinidius and Wolbachia spp. Hurungwe District is the only sleeping sickness focus in Zimbabwe and an increase in cases had been detected in years preceding this study. The objective of the study was to investigate the trypanosome species present in the tsetse population and their association with tsetse endosymbionts. This study was conducted as part of research into the relationship between trypanosomiasis, well-being and ecosystems by the Dynamic Drivers of Disease in Africa Consortium (DDDAC). The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c Anderson, N. The Royal (Dick) School of Veterinary Studies, University of Edinburgh Neil.Anderson@ed.ac.uk pointOfContact Goodwin, M. University of Edinburgh s1103812@sms.ed.ac.uk https://orcid.org/0000-0003-0823-4998 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Webber, F. University of Edinburgh s1671705@sms.ed.ac.uk https://orcid.org/0000-0002-1810-9640 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Hamill, L.C. The University of Edinburgh louisehamill85@gmail.com https://orcid.org/0000-0003-3992-326X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Anderson, N.E. The University of Edinburgh Neil.Anderson@ed.ac.uk https://orcid.org/0000-0001-7192-7717 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Shereni, W. Department of Livestock and Veterinary Services, Zimbabwe shereni2005@yahoo.com https://orcid.org/0000-0002-9427-4349 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Nyakupinda, L. Department of Livestock and Veterinary Services, Zimbabwe nyakupinda@gmail.com https://orcid.org/0000-0003-4768-0392 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Gwenhure, L.F. Central Veterinary Research and Diagnostic Laboratory, Zimbabwe lfgwenhure@yahoo.com https://orcid.org/0000-0001-8299-3082 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Macleod, E. The University of Edinburgh ewan.macleod@ed.ac.uk https://orcid.org/0000-0002-5517-0619 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian notPlanned Zimbabwe Hurungwe District GeoNames 2006-01-01 creation Dynamic Drivers of Disease in Africa Consortium (DDDAC) Sleeping sickness otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © The University of Edinburgh otherRestrictions © Central Veterinary Research and Diagnostic Laboratory, Zimbabwe otherRestrictions © Department of Livestock and Veterinary Services, Zimbabwe otherRestrictions If you reuse this data, you should cite: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c textTable English utf8 biota health 2014-02-01 2016-04-12 28.855 30.009 -16.701 -15.929 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/1e435dd3-ed93-4961-a136-ffa477731e1c Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/1e435dd3-ed93-4961-a136-ffa477731e1c.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.