221909cf-eac3-4a64-b1d0-3c1cdccdfe3c eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2024-01-11 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 In situ growth and physiological data from two Antarctic anemone species, Isotealia antarctica and Urticinopsis antarctica at Rothera Research Station (2020-2023) 2024-01-11 creation 2024-01-11 revision 2024-01-11 publication 2024-01-11 released 1.0 https://doi.org/10.5285/221909cf-eac3-4a64-b1d0-3c1cdccdfe3c doi GB/NERC/BAS/PDC/01816 https://data.bas.ac.uk/ Please cite this item as: Frontier, N., Marlow, J., Giles, A., Clark, M.S., Morley, S.A., & Peck, L.S. (2024). In situ growth and physiological data from two Antarctic anemone species, Isotealia antarctica and Urticinopsis antarctica at Rothera Research Station (2020-2023) (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/221909cf-eac3-4a64-b1d0-3c1cdccdfe3c To further our understanding of Antarctic predator growth and seasonal physiology, field growth rates were measured for two soft-bodied Antarctic anemone benthic predators, Isotealia antarctica and Urticinopsis antarctica, using in situ sampling of anemones on uniquely marked tiles from Rothera Research Station from 2020-2023. Ex situ measurements of oxygen consumption and seven-day faecal output were obtained from recently collected specimens in aquaria and compared between summer and winter. Winter physiological data for Antarctic species are rare, and we tested the hypothesis that generalist feeders or predators continue to feed during the winter. There is a dearth of basic life history and physiological data from Southern Ocean species, particularly from benthic sessile predators. This is an important data gap because species inhabiting the Southern Ocean live in a more temperature stable but seasonally varying environment than temperate and tropical counterparts. This work was supported by core funding from the NERC, UKRI, UK to the British Antarctic Survey. No credit. completed Frontier, Nadia British Antarctic Survey Researcher author Marlow, Joseph Scottish Association For Marine Science Researcher author Giles, Adriana British Antarctic Survey Researcher author Clark, Melody S British Antarctic Survey Researcher author Morley, Simon A British Antarctic Survey Researcher author Peck, Lloyd S British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Adaptation EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctic benthos Cnidaria buoyant weight ectotherms faecal egestion generalist feeders respirometry sessile predators soft-bodied predators theme West Antarctic Peninsula, Adelaide Island, Ryder Bay, Marguerite Bay, Rothera Re Antarctica South Cove, Cheshire Island, Backbay Lagoon Antarctica place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions Data are released under Open Government Licence V3.0: otherRestrictions None. url url crossReference doi doi crossReference url url crossReference textTable eng utf8 biota environment oceans -68.25571 -68.1255 -67.59595 -67.57069 2020-02-01 2023-09-30 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv bytes 51200 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=221909cf-eac3-4a64-b1d0-3c1cdccdfe3c WWW:LINK-1.0-http--link Get Data Download data download bytes 51200 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=221909cf-eac3-4a64-b1d0-3c1cdccdfe3c WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Barcoding: A ~10 mm snip of a single tentacle was collected from each anemone in the trial group (n = 28), which was preserved in ethanol for barcoding (574bp fragment of the 18s gene). DNA was extracted from tentacles using the DNeasy extraction kit (Qiagen) according to manufacturer's instructions. A 574bp fragment of the 18S rDNA gene was amplified using NSF4 (CTGGTTGATYCTGCCAGT) and NSR581 (ATTACCGCGGCTGCTGGC) primers using MyTaq PCR ready mix (Meridian Bioscience) and a PCR annealing temperature of 60°C. PCR products were sequenced using the NSR581 primer by Source Bioscience, with verification using NSF4, if required (Supplemental file S2). Initial species identities were confirmed by blastn sequence similarity searching against the NCBI nucleotide database. Assignment of different individual animals to each species was then performed using ClustalX. Buoyant weight: Buoyant weight was measured in the 45 experimental animals at time zero and at subsequent sampling points after 1 and 2 years on individuals recollected after these times. They were weighed after being gently removed from their tiles and cleaned of debris. Animals were placed on a scale suspended in ambient sea water and weights were recorded (± 0.001 g) using a Sartorius LA3200D balance. Respirometry: Respiration measurements were conducted in appropriately sized respiration chambers, chosen after preliminary trials aimed to identify chamber sizes and trial durations needed for approximately 20% reduction of oxygen in respirometers. Prior to respiration measurements, animals were placed within the chambers and left for 24 hours to habituate to the experimental conditions; chambers were left open to allow continual water exchange with the flow through aquarium, with a 1 mm mesh preventing the anemone escape. This gave anemones time to settle within their respirometer and reduce metabolic rates to routine levels. Prior to measuring oxygen consumption, chamber water was exchanged, all air bubbles were removed, and the lids sealed. In addition, three control chambers, without anemones, were sealed to account for any changes in background oxygen levels. Oxygen concentrations were then recorded within each chamber at 'time 0' (3 repeat measurements per chamber at each sampling interval) using a Fibox-3 oxygen system (PreSens GmBH with SP-PSt3-NAUspots and OxyView PST3-V5.31 b software). Measurements were collected at the start and at the end of a six-hour period, when oxygen saturation did not fall below 70% in any chamber. Prior to taking samples, chambers were gently inverted three times to mix the contents and ensure that three stable readings were recorded. Temperature was recorded from the aquarium tank that the respiration chambers were placed in. At the end of the respirometry measurements, the volume of each anemone was measured by displacement of the volume of sea water. This volume was then subtracted from the volume of the respirometer to calculate the final volume of water within the chamber from which oxygen was extracted. The Fibox-3 system uses oxygen spots inside chambers. During each calibration, the amplitude from each spot was checked to ensure it read over 10,000, a measure of the integrity of oxygen sensitive foil. Measurements were standardised by calibrating each chamber spot 24 hours post measurement. The Mean Sea Level (QFF) atmospheric pressure was recorded before measurements commenced. Spots in chambers were calibrated with 100 % saturated oxygen, which had been vigorously aerated for 30 minutes and then left to stand for 10 minutes. They were then calibrated for 0% oxygen using seawater with added sodium dithionite (2.5.%) at the ambient experimental temperatures. Faecal collection: Immediately after collection, anemones were placed in individual, standardised floating containers of 5632cm3, in the Bonner laboratory flow through aquar...(8) Data collection: Respirometry data was collected using Fibox-3 oxygen system, PreSens GmBH with SP-PSt3-NAUspots and OxyView PST3-V5.31 b software. Anemones were weighted on a Sartoritus LA320D balance Statistics were completed using R Studio version 4.3.1 Data quality: Respirometry data relies on the calibration of PreSens GmBH with SP-PSt3-NAUspots after each respirometry event. These spots are calibrated with 0 % oxygen water and 100 % saturated oxygen water. The Sartoritus LA320D balances are calibrated every year and are rotated. Losses of data_growth metrics_supplementalS4 (57KB), contain NA's because author was unable to obtain metrics for each individual anemone for ash free weights because anemone contain high water content and take a long time to dry. Furnace space was limited at Rothera. asNeeded completed