BGS Hosted Metadata
Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions
This dataset contains information relating to the survival and persistence of clinically relevant strains of E. coli on plastic and cotton, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model. This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1]. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
Simple
- Date (Publication)
- 2025-04-09
- Citation identifier
- https://catalogue.ceh.ac.uk/id/268cab8c-459c-4bf8-9b90-838c4d18efb2
- Citation identifier
- doi: / 10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
- Other citation details
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Ormsby, M.J., White, H.L., Metcalf, R., Oliver, D.M., Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre 10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Stirling Ormsby, M.J. Author University of Stirling Ormsby, M.J. Point of contact University of Stirling White, H.L.
Author University of Stirling Metcalf, R.
EMAIL NOT PROVIDED
Author University of Stirling Oliver, D.M. Author University of Stirling Quilliam, R.S. Author University of Stirling EMAIL NOT PROVIDED
Owner NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher
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GEMET - INSPIRE themes, version 1.0
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Human Health and Safety
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Ormsby, M.J., White, H.L., Metcalf, R., Oliver, D.M., Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Begin date
- 2023-01-01
- End date
- 2023-06-28
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/268cab8c-459c-4bf8-9b90-838c4d18efb2 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/268cab8c-459c-4bf8-9b90-838c4d18efb2.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Biofilm formation, persistence and pathogenicity of E. coli were investigated through controlled laboratory experiments. Biofilms were generated on various materials in nutrient-rich and surface water environments, quantified using crystal violet staining, and analysed in triplicate. Persistence was assessed by recovering E. coli from biofilms over 28 days, followed by serial dilution, and selective plating. Pathogenicity was evaluated using Galleria mellonella larvae, with standardised inoculation protocols and survival monitoring for 72 hours. Data underwent thorough validation at each stage, including replication, and selective media confirmation, ensuring high reliability and accuracy before submission.
Metadata
- File identifier
- 268cab8c-459c-4bf8-9b90-838c4d18efb2 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-04-10T15:32:18
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
