27da4400-9835-4241-9df9-a3b598f8d274 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:23:47 UK GEMINI 2.3 OSGB 1936 / British National Grid Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013) 2017-09-20 publication 2013-06-15 creation https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274 10.5285/27da4400-9835-4241-9df9-a3b598f8d274 doi: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre 10.5285/27da4400-9835-4241-9df9-a3b598f8d274 The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274 MacDonald, H. Cardiff University Hannah.c.burton@gmail.com pointOfContact MacDonald, H.C. Cardiff University Hannah.c.burton@gmail.com author Bruford, M.W. Cardiff University BrufordMW@cardiff.ac.uk author Ormerod, S. Cardiff University Ormerod@cardiff.ac.uk https://orcid.org/0000-0002-8174-302X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Jones, T.H. Cardiff University JonesTH@cardiff.ac.uk author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher Cardiff University EMAIL NOT PROVIDED owner notPlanned Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Amphinemura sulcicollis Isoperla grammatica Baetis rhodani Wikidata 2012-10-29 creation Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS) otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © Cardiff University otherRestrictions If you reuse this data, you should cite: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274 textTable English utf8 biota 2012-06-15 2013-06-15 -4.073 -3.08 51.921 53.123 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/27da4400-9835-4241-9df9-a3b598f8d274 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.