34df30f2-3163-4c11-8743-3732e49220fb English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-01-30T08:45:47 UK GEMINI 2.3 OSGB 1936 / British National Grid High spatial resolution seasonal distributions of faecally-derived waterborne and sediment bacteria in standing waters, Glasgow, UK, 2016-2017 2022-06-27 publication https://catalogue.ceh.ac.uk/id/34df30f2-3163-4c11-8743-3732e49220fb 10.5285/34df30f2-3163-4c11-8743-3732e49220fb doi: Pattison, Z., Quilliam, R.S., Oliver, D., Willby, N.J. (2022). High spatial resolution seasonal distributions of faecally-derived waterborne and sediment bacteria in standing waters, Glasgow, UK, 2016-2017. NERC EDS Environmental Information Data Centre 10.5285/34df30f2-3163-4c11-8743-3732e49220fb This dataset contains information about water quality based on faecal indicators at 15 lakes in the Greater Glasgow conurbation, Scotland. Lakes were sampled in winter (2016/17) and summer (2017) with faecal indicators being quantified at high spatial resolution (up to 60 points per lake depending on water body size) in sediment and water from each lake. Faecal indicators were quantified based on standard dilution, membrane filtration and incubation for water, and incubation in bacteria-specific broth, followed by plating and incubation for sediment extracts. Contextual information about water quality, water bird densities, and land cover in different buffer sizes is also provided for each lake. The data were collected to investigate how connectivity and stressors interact to determine biodiversity and ecosystem function in freshwaters. The work was supported by the Natural Environment Research Council grant NE/N006437/1 (Hydroscape: connectivity x stressor interactions in freshwater habitats) Full details about this dataset can be found at https://doi.org/10.5285/34df30f2-3163-4c11-8743-3732e49220fb Willby, N.J. University of Stirling n.j.willby@stir.ac.uk https://orcid.org/0000-0002-1020-0933 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Pattison, Z. University of Newcastle Zarah.pattison@newcastle.ac.uk https://orcid.org/0000-0002-5243-0876 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Quilliam, R.S. University of Stirling richard.quilliam@stir.ac.uk https://orcid.org/0000-0001-7020-4410 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Oliver, D. University of Stirling david.oliver@stir.ac.uk https://orcid.org/0000-0002-6200-562X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Willby, N.J. University of Stirling n.j.willby@stir.ac.uk https://orcid.org/0000-0002-1020-0933 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Stirling n.j.willby@stir.ac.uk owner Human Health and Safety Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication microbiology urban area GEMET - Concepts, version 4.1.3 2019-01-28 creation Water quality Glasgow faecal indicators spatial variation connectivity otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Pattison, Z., Quilliam, R.S., Oliver, D., Willby, N.J. (2022). High spatial resolution seasonal distributions of faecally-derived waterborne and sediment bacteria in standing waters, Glasgow, UK, 2016-2017. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/34df30f2-3163-4c11-8743-3732e49220fb textTable 1 English utf8 biota health 2016-01-01 2017-02-28 -4.603 -3.834 55.674 55.983 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/34df30f2-3163-4c11-8743-3732e49220fb Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/34df30f2-3163-4c11-8743-3732e49220fb.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Sub-surface water samples and cores of the upper 5cm of sediment obtained from multiple geolocated points within each of 15 water bodies via boat-based sampling. For consistency all sites were sampled 3 hours after sunrise in summer (July) and on ice-free days in winter (Dec/Jan). Sampling intensity was dependent on water body size. Bird distribution and numbers were recorded for an hour from a suitable vantage point at the same time of sampling. A separate water sample was collected from a mid-point in each site for laboratory analysis of metals, nutrients and other standard determinands (Thermo iCap 6000 Series or Dionex DX-120). Dissolved oxygen, pH, electrical conductivity and temperature were measured in the field using a HACH multiprobe. Standard water body characteristics (area, elevation, perimeter etc) are sourced from the U.K. Lakes Portal (https://uklakes.ceh.ac.uk/) Water samples for microbial analysis were stored at 4 C and processed within 6 h of collection. 100ml samples at three dilutions were filtered through 0.45-m membrane filters, placed onto the surface of Membrane Lactose Glucoronide Agar (MLGA) agar. Plates were incubated at 37 deg C and counted after 24 h. Counts are reported as colony forming unit (CFU) per 100 ml water. A mixture of sediment (~2.5 g) and 50ml Phosphate buffered saline (PBS) was used to enumerate E. coli in the sediment. All sediment dilutions were made by placing samples in sterile PBS and shaking them. Each dilution was spread using disposable sterile loops onto MLGA agar plates and incubated at 37 deg C for 24 hours before counting colonies. To quantify abundance of Campylobacter, I. Enterococci, E. coli 0157 and E.coli in sediment a single composite 1g subsample was placed into a sterilised glass vial of 100ml LB broth. A 100ug sample of the aliquot of each sample was spread over the surface of agar plates with a disposable sterile loop and incubated following isolation and incubation protocol for each type of bacteria. Colonies were counted, and results are reported as CFU per g oven-dried soil. Data were added to an Excel spreadsheet and exported as a .csv file for deposit into the EIDC.