391c0294-07f1-4856-b592-428bd44055ca English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:25:58 UK GEMINI 2.3 WGS 84 Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017) 2019-04-23 publication https://catalogue.ceh.ac.uk/id/391c0294-07f1-4856-b592-428bd44055ca 10.5285/391c0294-07f1-4856-b592-428bd44055ca doi: Goodall, T.I., Griffiths, R.I., Blaud, A., Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre 10.5285/391c0294-07f1-4856-b592-428bd44055ca The data consist of nitrogen gene data, soil biodiversity indices and microbial community composition for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located in the United Kingdom and collected between April 2017 and August 2017. The sites were Rothamsted Research at North Wyke in Devon and Bangor University at Henfaes Research Station in North Wales. At each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate a nitrification inhibitor. Soil samples were taken within two weeks of digestate application and shortly before winter wheat harvest. Soil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. Soil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford). Soil physico-chemical parameters were measured on the same soil samples and are presented in a related dataset. https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab Data was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC - Ref BB/N013468/1 Full details about this dataset can be found at https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca Reinsch, S. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-4649-0677 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Goodall, T.I. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Griffiths, R.I. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Blaud, A. Rothamsted Research aimeric.blaud@rothamsted.ac.uk https://orcid.org/0000-0003-0860-8080 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Abadie, M. Rothamsted Research maider.abadie@rothamsted.ac.uk author Clark, I.M. Rothamsted Research ian.clark@rothamsted.ac.uk https://orcid.org/0000-0002-1589-5420 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Hirsch, P.R. Rothamsted Research comms@rothamsted.ac.uk author Carswell, A.M. Rothamsted Research alison.carswell@rothamsted.ac.uk https://orcid.org/0000-0003-0580-6698 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Misselbrook, T.H. Rothamsted Research tom.misselbrook@rothamsted.ac.uk https://orcid.org/0000-0002-4594-3606 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Sánchez-Rodríguez, A.R. Bangor University antonio.sanchez@uco.es https://orcid.org/0000-0001-8734-2035 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Chadwick, D.R. Bangor University d.chadwick@bangor.ac.uk author Jones, D.L. Bangor University d.jones@bangor.ac.uk https://orcid.org/0000-0002-1482-4209 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Reinsch, S. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-4649-0677 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication operational taxonomic unit diversity index bacterial community composition winter wheat nitrogen fertiliser digestate otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © Rothamsted Research otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions © Bangor University otherRestrictions If you reuse this data, you should cite: Goodall, T.I., Griffiths, R.I., Blaud, A., Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca textTable English utf8 farming 2017-04-01 2017-10-31 -4.022 -3.892 50.767 53.241 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/391c0294-07f1-4856-b592-428bd44055ca Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/391c0294-07f1-4856-b592-428bd44055ca.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs'). Soil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific). Quantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018. Extracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012. All results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.