3d468857-f5d0-4dc4-88f3-6be6df19608b English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:26:12 UK GEMINI 2.3 WGS 84 Calluna vulgaris root length and fungal colonisation data from the Climoor long-term climate change experiment in Clocaenog forest, UK (2015) 2019-10-11 publication https://catalogue.ceh.ac.uk/id/3d468857-f5d0-4dc4-88f3-6be6df19608b 10.5285/3d468857-f5d0-4dc4-88f3-6be6df19608b doi: White, N., Seaton, F.M., Reinsch, S., Smith, A.R., Brooks, M.R., Emmett, B.A. (2019). Calluna vulgaris root length and fungal colonisation data from the Climoor long-term climate change experiment in Clocaenog forest, UK (2015). NERC Environmental Information Data Centre 10.5285/3d468857-f5d0-4dc4-88f3-6be6df19608b This dataset contains root length, biomass and fungal colonisation data for Calluna vulgaris from control, drought and warming treated soils from the long term climate change experiment in Clocaenog forest. Soil samples were collected from the climate change experiment in Northeast Wales during April 2015. Roots were separated from the soil, their length and biomass measured and then analysed using microscopy for Ericoid mycorrhizae (ErM) and dark septate endophyte (DSE) colonisation of Calluna vulgaris. The experimental field site consists of three untreated control plots, three plots where the plant canopy air is artificially warmed during night time hours and three plots where rainfall is excluded from the plots at least during the plants growing season (March to September). The Climoor field experiment intends to answer questions regarding the effects of warming and drought on ecosystem processes and has been running since 1999. The root length and fungal colonisation data aims to understand how changes in soil hydrological and chemical properties have influenced Calluna vulgaris rooting behaviour and interactions with the soil microbiome. This work was supported by the Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCAPE programme delivering National Capability. Full details about this dataset can be found at https://doi.org/10.5285/3d468857-f5d0-4dc4-88f3-6be6df19608b Seaton, F. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact White, N. Bangor University nwhite710@hotmail.com author Seaton, F.M. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0002-2022-7451 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Reinsch, S. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-4649-0677 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Smith, A.R. Bangor University a.r.smith@bangor.ac.uk https://orcid.org/0000-0001-8580-278X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Brooks, M.R. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0002-7439-0393 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Emmett, B.A. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0002-2713-4389 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Calluna vulgaris Wikidata 2012-10-29 creation fungus soil drought climate change GEMET - Concepts, version 4.1.3 2019-01-28 creation Climate and climate change North Wales Clocaenog UK-SCAPE Warming heather ling otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions © Bangor University otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions If you reuse this data, you should cite: White, N., Seaton, F.M., Reinsch, S., Smith, A.R., Brooks, M.R., Emmett, B.A. (2019). Calluna vulgaris root length and fungal colonisation data from the Climoor long-term climate change experiment in Clocaenog forest, UK (2015). NERC Environmental Information Data Centre https://doi.org/10.5285/3d468857-f5d0-4dc4-88f3-6be6df19608b textTable 100 English utf8 biota 2015-04-01 2015-04-01 -3.479 -3.391 53.033 53.077 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/sd/3d468857-f5d0-4dc4-88f3-6be6df19608b.zip Supporting information Supporting information available to assist in re-use of this dataset information https://data-package.ceh.ac.uk/data/3d468857-f5d0-4dc4-88f3-6be6df19608b Download the data Download a copy of this data download dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Soil cores of 8cm diameter and depth were collected from each plot near the base of Calluna vulgaris on the 1st April 2015, then transported back to Bangor at 4°C. Cores were cut from the top into 1 cm deep subsections. Each subsection was soaked and agitated to break up the root/soil clumps. Roots confidently identified as C. vulgaris were removed by hand and thoroughly washed in tap water. Necrotic or rotting roots were discarded. WinRHIZO version 3.2 was used to measure the length and diameter of cleaned subsection roots on a flatbed scanner. Roots were positioned without overlapping, submerged in 5 mm tap water to improve scanning accuracy. Acquisition parameters were set using the TWAIN interface in professional mode: positive film, 24 bit and 300 dpi. Post scanning, ten of the finest roots were manually selected from each subsection for microscopic investigation. The remaining roots were oven dried at 70°C for 24 hours, producing dry weight data for those < and > 2mm in diameter. All core fragments for microscopic assessment were soaked over 20 hours in 10% Potassium hydroxide (KOH). Roots were thoroughly rinsed in deionised water and heated in a water bath at 90°C for 15 minutes in 5% vinegar-ink solution. Roots were rinsed in three changes of tap water, acidified and de-stained by soaking in tap water with a few drops of vinegar for a further 20 minutes. A compound microscope was used to estimate proportional colonisation using the magnified intersection technique, with a scale bar cuticle instead of cross-hair and at a 40x magnification. Roots were cut approximately 1-2 cm in length, with 2 mm passes made along each root length. All cortical cells were examined for Ericoid mycorrhizae (ErM) and dark septate endophyte (DSE), working through the plane of focus. Each interval was categorised based upon ErM colonisation into 0 %, < 1 %, < 10 %, < 50 %, > 50 % and > 90 % colonisation. Data were transferred into an Excel spreadsheet. Data were exported as a comma separated value file for ingestion into the EIDC.