3ef187b8-3471-42ab-bc5d-d7e5e6f5d575 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2023-04-28 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 1.0 2023-04-28 creation 2023-04-28 revision 2023-04-28 publication 2023-04-28 released 1.0 https://doi.org/10.5285/3ef187b8-3471-42ab-bc5d-d7e5e6f5d575 doi GB/NERC/BAS/PDC/01741 https://data.bas.ac.uk/ Please cite this item as: Newsham, K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3ef187b8-3471-42ab-bc5d-d7e5e6f5d575 Data on the growth rates and specific cellulase activities of 10 isolates of the fungus Pseudogymnoascus roseus from sub- and maritime Antarctica, exposed in vitro to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius and their constant mean temperatures of 9.0 degrees Celsius, 10.7, 12.0 or 13.1 degrees Celsius, respectively. The 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia, The Backslope on Signy Island, Walton Terraces on Leonie Island and Mars Oasis on Alexander Island. Funding: UK Natural Environment Research Council (core funding to the British Antarctic Survey). *** PLEASE BE ADVISED TO USE VERSION 2.0 OF THIS DATASET *** This dataset has been superseded by Version 2.0 (https://data.bas.ac.uk/full-record.php?id=GB/NERC/BAS/PDC/01807 - see 'Related Data Set Metadata' link below). Version 2.0 has been updated to include additional data from a third experiment and to update the metadata accordingly No credit. completed Newsham, Kevin K. British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Fungi theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctic heatwaves beta-glucosidase cellulase fluctuating temperature growth rate psychrotrophy soil fungi theme Lewis Pass, South Georgia The Backslope, Signy Island Walton Terraces, Leonie Island Mars Oasis, Alexander Island place Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions Data released under Open Government Licence V3.0: Use the following citation: Newsham, K. K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3EF187B8-3471-42AB-BC5D-D7E5E6F5D575 otherRestrictions No restrictions apply. url url crossReference url url crossReference textTable eng utf8 biota -68.35778 -36.49833 -71.87833 -54.26472 2021-09-01 2023-03-31 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/csv text/plain bytes 14336 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=3ef187b8-3471-42ab-bc5d-d7e5e6f5d575 WWW:LINK-1.0-http--link Get Data Download data download bytes 14336 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=3ef187b8-3471-42ab-bc5d-d7e5e6f5d575 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium. Isolates were then inoculated onto soil extract medium in Petri dishes, which were incubated at temperatures fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius, and at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius, respectively. The constant temperatures were the means of the fluctuating temperatures (i.e., 9.0 degrees Celsius was the mean of the temperatures fluctuating from 2-15 degrees Celsius, 10.7 degrees Celsius was the mean of the temperatures fluctuating from 2-18 degrees Celsius, 12.0 degrees Celsius was the mean of the temperatures fluctuating from 2-21 degrees Celsius, and 13.1 degrees Celsius was the mean of the temperatures fluctuating from 2-24 degrees Celsius). Four replicate dishes per isolate were incubated at each temperature. Radial hyphal extension rates of colonies (referred to as growth rates) were recorded in one experiment at 15 x magnification under a dissecting microscope at 0-48 hours and were expressed as mm / day. Growth rates were also measured in another experiment, again at 15 x magnification, every 3-5 days at 7-28 days after inoculation, and were calculated by regressing colony radii against time (r2 of all fits more than or equal to 95 percent). At 28 d, extracellular beta-glucosidase (cellulase) was extracted from colonies in 10 mM phosphate buffer. Protein concentrations in the buffer were measured using a Micro BCA Protein Assay (Thermofisher). Cellulase activity was measured and expressed as nmol of p-nitrophenol released from 4-nitrophenyl-beta-D-glucopyranoside min-1 micro-grammes-1 protein (Misiak et al., 2021). Data collection: Micro BCA Protein Assay (Thermofisher) Data quality: Data have not been cleaned. Data losses, indicated by NaN were caused by laboratory errors. Note that the accuracy of growth rate data measured at 12 degrees Celsius and 2-21 degrees Celsius over 0-48 hours was lower than for other growth rate data. asNeeded completed