4434a27d-5288-4f2e-88ac-4b1372e4d073 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:15:44 UK GEMINI 2.3 Drosophila C virus genomes, Cambridge (2017) 2018-01-24 publication https://catalogue.ceh.ac.uk/id/4434a27d-5288-4f2e-88ac-4b1372e4d073 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073 doi: Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073 The resource consists of genome sequence data for the Drosophila C virus that has been serially passaged through different species of Drosophila in the laboratory. The genomes were sequenced and aligned to the reference genome. The frequency of variants at both biallelic and triallelic sites was then calculated. We also generated a phylogeny of the species involved using published data. This data was generated to understand how viruses adapt to new host species by Francis Jiggins and his co workers. The work was carried out between July 2016 and September 2017 and was funded by NERC under award reference NE/L004232/1 Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073 Jiggins, F. University of Cambridge fmj1001@cam.ac.uk pointOfContact Jiggins, F.M. University of Cambridge fmj1001@cam.ac.uk https://orcid.org/0000-0001-7470-8157 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Longdon, B. University of Exeter b.longdon2@exeter.ac.uk https://orcid.org/0000-0001-6936-1697 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher University of Cambridge EMAIL NOT PROVIDED owner notPlanned evolution disease virus GEMET - Concepts, version 4.1.3 2019-01-28 creation drosophila DCV Drosophila C virus disease emergence host shift otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © Cambridge University otherRestrictions If you reuse this data, you should cite: Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073 English utf8 health 2016-01-01 2017-09-01 Comma-separated values (CSV) BAM NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/sd/4434a27d-5288-4f2e-88ac-4b1372e4d073.zip Supporting information Supporting information available to assist in re-use of this dataset information https://catalogue.ceh.ac.uk/datastore/eidchub/4434a27d-5288-4f2e-88ac-4b1372e4d073 Download the data Download a copy of this data fileAccess nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Each species of Drosophila was infected by dipping a needle in virus suspension. After 3 days the fly was then homogenized and the process repeated for 8-10 passages. At the end of this process, Ribonucleic acid (RNA) was extracted, the viral genome amplified by Polymerase chain reaction (PCR) and Nextera libraries constructed. These were sequenced on n Illumina sequencer, the sequences quality trimmed and aligned to the reference genome to generate the BAM file in this dataset. We then called variants to generate the allele frequency CSV files. Full details of these steps are provided in the supporting documentation. NB: these data are not adequately described in that there are no column descriptions for the csv files.