46c23be6-63ba-400d-859b-050dcb207898 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-01-05T08:38:21 UK GEMINI 2.3 Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature 2025-04-17 publication https://catalogue.ceh.ac.uk/id/46c23be6-63ba-400d-859b-050dcb207898 10.5285/46c23be6-63ba-400d-859b-050dcb207898 doi: Hellmann, E., Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre 10.5285/46c23be6-63ba-400d-859b-050dcb207898 This dataset contains information on how variations on temperature and photoperiod affects poplar seedling growth in Eco chambers. Plants were propagated on sterile media (1/2MS, 0.7% Agar, 1mg/L IAA) by cutting the shoot tip and 3-4 leaves and placing them in a weck jar on new medium. Once the explants rooted after incubation at 200 mmol light, 21C day/18C night, long day conditions (16:8) and ambient humidity, they were transplanted onto soil (6:2:1 Peat-free: sand-vermiculite, fertilizer: 4 g/L Osmocote exact standard 15-9-12) and grown first under protective covers and then open in 24C, long day conditions (LD), 65% humidity, 350mmol light (experiments R1-3) or transferred into the Eco chamber (temperature as indicated in experiment, 80-98% humidity, 350mmol LED light, long day conditions). After 3-4 weeks, plants of R1-R3 were transferred in the conditions described in the experiment (R1: 24C SD vs 15C LD; R2: 15C SD vs 15C LD; R3: 15C LD vs 24C LD) and grown for a month before moving them into 5C low light conditions which resulted in senescence and leaf drop. Eco chamber plants were grown in the Eco chamber for about four weeks and after not showing senescence, harvested directly after the experiment without cold incubation. All plants were measured in height and diameter (6cm above soil), leaf number and area regularly and gas exchanged was measured with a TARGAS-1 instrument (Hansatech) at 500mmol light intensity. After the experiment, cross-sections were taken and embedded in resin for further microscopic analysis (data not ready and not included). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898 Hellmann, E. Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde Eva.hellmann@slcu.cam.ac.uk https://orcid.org/0000-0002-4591-2973 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Hellmann, E. Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde Eva.hellmann@slcu.cam.ac.uk https://orcid.org/0000-0002-4591-2973 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Friend, A.D. Department of Geography, Cambridge Adf10@cam.ac.uk https://orcid.org/0000-0002-9029-1045 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Cambridge EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication photosynthesis temperature GEMET - Concepts, version 4.1.3 2019-01-28 creation Poplar day-length growth otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Hellmann, E., Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898 English utf8 biota 2023-01-01 2023-12-31 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/46c23be6-63ba-400d-859b-050dcb207898 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/46c23be6-63ba-400d-859b-050dcb207898.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The data have been generated using calipers, a laser distance meter, Image J for leaf area and a TARGAS-1 instrument for photosynthetic gas exchange. The data have been averaged between 20-25 plants and the standard deviation calculated.