BGS Hosted Metadata
Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Simple
- Date (Publication)
- 2025-02-12
- Citation identifier
- https://catalogue.ceh.ac.uk/id/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Citation identifier
- doi: / 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Other citation details
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Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Manchester
Connolly, J.A. Author University of Manchester
Del Carratore, F. Author University of Manchester
Schmidt, K.
Author University of Minnesota
Bisesi, A.
Author University of Minnesota
Martinson, J.N.V.
Author University of Manchester
Chua, J.
Author University of Minnesota
Kuhs, M.
Author University of Minnesota
Boneza, M.
Author University of Minnesota
Heinsch, S.
Author University of Minnesota
Kinkel, L. Author University of Minnesota
Smanski, M.
Author University of Minnesota
Harcombe, W.R.
Author University of Manchester
Breitling, R. Author University of Manchester
Takano, E. Author University of Manchester
Owner NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher University of Manchester
Connolly, J.A.
Point of contact
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GEMET - INSPIRE themes, version 1.0
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Species Distribution
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Distribution format
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Name Version .mzML
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://catalogue.ceh.ac.uk/datastore/eidchub/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
Metadata
- File identifier
- 475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-03-21T12:34:34
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
