BGS Hosted Metadata
Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
This dataset contains features detected by untargeted metabolomics analysis of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains the mass-to-charge (m/z) and retention time of identified peaks, and their ion intensity in each sample. The relative abundance of these identified features was later compared between pairs of interacting strains to gain insight into changes in their metabolism. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
Simple
- Date (Publication)
- 2024-11-21
- Date (Creation)
- 2024-10-21
- Citation identifier
- https://catalogue.ceh.ac.uk/id/4921f306-5efa-4bf7-a15e-a157c2665889
- Citation identifier
- doi: / 10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
- Other citation details
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Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2024). Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre 10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Manchester Connolly, J.A. Author University of Manchester Connolly, J.A. Point of contact University of Manchester Del Carratore, F. Author University of Manchester Schmidt, K.
Author University of Minnesota Bisesi, A.
Author University of Minnesota Martinson, J.N.V.
Author University of Manchester Chua, J.
Author University of Minnesota Kuhs, M.
Author University of Minnesota Boneza, M.
Author University of Minnesota Heinsch, S.
Author University of Minnesota Kinkel, L. Author University of Minnesota Smanski, M.
Author University of Minnesota Harcombe, W.R.
Author University of Manchester Breitling, R. Author University of Manchester Takano, E. Author NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher University of Manchester EMAIL NOT PROVIDED
Owner
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2024). Features detected by untargeted metabolomic analysis of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Environment
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/4921f306-5efa-4bf7-a15e-a157c2665889 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/4921f306-5efa-4bf7-a15e-a157c2665889.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Noise removal, signal filtering and peak matching was performed in the open source mzMatch software. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
Metadata
- File identifier
- 4921f306-5efa-4bf7-a15e-a157c2665889 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-03-21T12:34:20
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
