BGS Hosted Metadata
Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms
This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Simple
- Date (Publication)
- 2024-05-13
- Date (Creation)
- 2023-03-10
- Citation identifier
- https://catalogue.ceh.ac.uk/id/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Citation identifier
- doi: / 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Other citation details
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Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Point of contact
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Organisation name Individual name Electronic mail address Role Jazz Pharmaceuticals
Hahn, C.Z. Author University of Liverpool Airey, G.A.
Author Woodland Trust Crawford, J.A.
Author Lancaster University Sayer, E.J. Author Lancaster University Stevens, C.J. Author University of Liverpool Paterson, S. Author University of Liverpool Whitlock, R. Author University of Liverpool Whitlock, R. Point of contact NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher University of Liverpool EMAIL NOT PROVIDED
Owner
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Wikidata
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GEMET - Concepts, version 4.1.3
- Keywords
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soil bacteria
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experimental evolution
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soil ecology
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Environment
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/4b27e5d5-2ec4-4b72-9939-ab66c2818612 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/4b27e5d5-2ec4-4b72-9939-ab66c2818612.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Bacterial isolates from BCCIL (twelve from each of genera Bacillus and Pseudomonas) were raised in liquid media and used to inoculate sterilised soil in kilner jars (approx 100 ml soil in 500 ml jars). Each isolate of each bacterial taxon was inoculated into five replicate microcosms per moisture treatment (drought and control) and microcosms were assigned random positions within four experimental blocks. Microcosms in the control treatment were topped up weekly to a volumetric water content of 40%. Those in the drought treatment were subjected to a series of four simulated droughts lasting six weeks, where watering was not carried out. Soil moisture content determination and watering was carried out by assessing the mass of each kilner jar and the dried soil it initially contained, allowing controlled watering to the target soil water content. Substrate utilisation profiling was carried out by taking soil samples from the microcosms at the end of the final drought cycle, which were amended with different substrates (water, glucose, cellulose, artificial root exudate)in MicroResp(TM) colourimetric microplates, before CO2 efflux detection on a FLUOstar Omega spectrophotometer (BMG Labtech, Ortenberg, Germany). q-PCR was carried out by taking soil samples from the microcosms in the experimental evolution. RNA was extracted from the soil samples and amplified using qPCR primers specific to the isolates which had been used to inoculate the microcosms.
Metadata
- File identifier
- 4b27e5d5-2ec4-4b72-9939-ab66c2818612 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-11-13T16:19:54
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
