4d781314-b2a6-47fe-aab9-4364a9022e44 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2021-01-26 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Phytoplankton enumeration and biomass calculations from samples collected in the Fram Strait, Greenland Basin and Svalbard region during 2017-2018 2021-01-26 creation 2021-01-26 revision 2021-01-26 publication 2021-01-26 released 1.0 https://doi.org/10.5285/4d781314-b2a6-47fe-aab9-4364a9022e44 doi GB/NERC/BAS/PDC/01461 https://data.bas.ac.uk/ NE/P006213/1 award Please cite this item as: Mitchell, E., & Pond, D. (2024). Phytoplankton enumeration and biomass calculations from samples collected in the Fram Strait, Greenland Basin and Svalbard region during 2017-2018 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/4d781314-b2a6-47fe-aab9-4364a9022e44 Collection and preservation of open ocean water samples from stations along a transect up the east coastline of Greenland and then across the Fram Strait to Svalbard during May 2018 & August 2019. Two cruises in total to cover annual changes, both on board the James Clark Ross (RRS). A standard CTD cast was deployed to collect the samples, depths were surface and the chlorophyll maximum, selected to support zooplankton net sampling and other on board experiments. Research assistants from SAMS (Scottish Association for Marine Science) were responsible for the sample collection on JR17-005, Dave Pond of Stirling University was responsible for collection on JR18-007. Elaine Mitchell of SAMS was responsible for the sample analysis and data processing. Funding was provided by the DIAPOD - NERC Thematic grant - Changing Arctic Ocean (CAO) programme - NE/P006213/1 No credit. completed Mitchell, Elaine Scottish Association For Marine Science Researcher author Pond, David University of Stirling Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Biomass EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Phytoplankton EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher C:N ratio Fram Strait biomass enumeration phytoplankton theme Greenland Basin, Norsk Trough, Fram Strait, Arctic Kongsfjord Basin Arctic place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions None. url url crossReference url url crossReference url url crossReference url url largerWorkCitation url url crossReference textTable eng utf8 biota environment oceans -5.46434 10.84316 75.3356 80.28329 2018-05-10 2018-06-08 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv bytes 364544 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=4d781314-b2a6-47fe-aab9-4364a9022e44 WWW:LINK-1.0-http--link Get Data Download data download bytes 364544 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=4d781314-b2a6-47fe-aab9-4364a9022e44 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: 2 cruises: JR17005 / JR18007 Seawater was collected from two depths from a standard environmental CTD cast as close to net collections. Sampling depths were selected based on the PAR irradiance readings from the CTD at the surface of the water (approx. 2m) after being initially stabilised at 10m and bought back to the surface. For phytoplankton samples two depths were selected - Surface and the Chlorophyll maximum. 10L acid washed carboys and acid washed tubing were used to collect the water samples. The carboys were stored in either the cold room or on deck in a low light area. Location of the collected water for storage until processing was dependent on the temperature of the surface water at the point of collection. 4ml of Lugol's solution, 1% (final concentration) was dispensed into 500ml glass amber bottles. 400ml of water sample was added, and samples stored in the cold room at 4-5°C. Laboratory analysis: Before observation through a light microscope, samples fixed with 1% Lugol's iodine were settled using a Hydro-Bios glass plate chamber and 50ml tower. The inverted samples were poured into the tower, so it was just over full. A glass coverslip was placed over the top of the tower, ensuring there were no bubbles in the column. Vacuum pressure holds the chamber set up together. The base plate and tower were then carefully lifted into a tray and left to settle for a minimum of 20 hours. After adequate settling time, the chamber was removed from the tray and distilled water was dropped either side of the tower base. The tower was removed by gently pushing it across the base plate, followed by a square coverslip, ensuring there was no gap to minimise bubbles. The baseplate and tower were then lifted carefully, and the remaining contents were emptied into a waste beaker. The base plate was dried, ready for microscopy. The waste beaker, tower and tower cover slip were washed in a sink with hot water. The prepared base plates were transferred to an inverted light microscope, the light source was set to a preferred intensity and viewed using a x20 objective, Phase2 contrast conditions. Where cells were in high abundance, a minimum of 100 cells were counted for statistical reasons, which was achieved by determining a set number of fields of view. Where cells are rare or in low abundance, the whole of the chamber was counted. A calibrated eyepiece graticule was used to size phytoplankton cells from the chlorophyll maximum samples only. Imaging software confirmed eyepiece sizing on a select number of cells. Conversion of cell sizes to biomass volumes was achieved using geometric calculations - specific for each cell type. Calculations take into consideration the shrinkage effect of the fixative on the cells to give a live volume. From this live volume C:N ratios were calculated. Cell enumeration results for each species at each station are provided as - cell numbers/litre. Cell sizes for biomass calculations are provided as - Length in µm, Breadth in µm. Biomass volumes are provided as - pgC per cell and Total µgC for each station. Data collection: Standard CTD and associated software used to determine ocean parameters and define collection depths on board the ship. Sample analysis conducted on a Zeiss S100 inverted microscope by Elaine Mitchell. Axio Imager software version 4.1 was used to take images and confirm eyepiece graticule sizing. Data from microscope analysis was entered into Comma Separated Value (csv) files for cell enumerations, biomass and C:N ratio calculations. Data quality: Sizing of cells for biomass calculations were done using an eye piece graticule on the microscope, a maximum of 30 cells of each type were sized for each sample - where possible - from the chlorophyll maximum depth. A small number of images were taken and the manual eyepiece measurements were compared to the measurements taken using the Axio imager software and in each case they were almost the same if you rounded up the imaging sizes to the nearest micron. David Pond at Stirling University checked the data for errors/alterations before submission. asNeeded completed