Date (Publication)

2019-01-10

Citation identifier

https://catalogue.ceh.ac.uk/id/500193f7-2653-4696-8224-276a734ed6ab

Citation identifier

doi: / 10.5285/500193f7-2653-4696-8224-276a734ed6ab

Other citation details

Ullah, S., Glover, L., Percival, A. (2019). Rates of fluorescein diacetate hydrolysis, phenol oxidation and potential denitrification in sand and gravel sediments from an agriculturally-impacted stream. NERC Environmental Information Data Centre 10.5285/500193f7-2653-4696-8224-276a734ed6ab

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of Birmingham/University of Keele	Ullah, S.	s.ullah@bham.ac.uk	Author
University of Keele	Glover, L.	EMAIL NOT PROVIDED	Author
University of Keele/Dublin Institute of Technology	Percival, A.	EMAIL NOT PROVIDED	Author
University of Birmingham	Ullah, S.	s.ullah@bham.ac.uk	Point of contact
NERC Environmental Information Data Centre		info@eidc.ac.uk	Publisher
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Custodian

GEMET - INSPIRE themes, version 1.0

• Environmental Monitoring Facilities

GEMET - Concepts, version 4.1.3

• denitrification
• agriculture
• gravel
• sand
• sediment
• nitrogen cycle
• carbon cycle

Keywords

• streambed

• substrate type

• microbial activity

• phenol oxidation

• fluorescein diacetate hydrolysis activity

• extracellular phenol oxidase activity

Access constraints

Other restrictions

Other constraints

no limitations

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This resource is made available under the terms of the Open Government Licence

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© Natural Environment Research Council

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If you reuse this data, you should cite: Ullah, S., Glover, L., Percival, A. (2019). Rates of fluorescein diacetate hydrolysis, phenol oxidation and potential denitrification in sand and gravel sediments from an agriculturally-impacted stream. NERC Environmental Information Data Centre https://doi.org/10.5285/500193f7-2653-4696-8224-276a734ed6ab

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Inland waters

Begin date

2015-06-01

End date

2015-06-30

Unique resource identifier

WGS 84

Distribution format

Name	Version
Comma-separated values (CSV)

Distributor contact

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Distributor

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/sd/500193f7-2653-4696-8224-276a734ed6ab.zip	Supporting information

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/data/500193f7-2653-4696-8224-276a734ed6ab	Download the data

Hierarchy level

Dataset

Other

dataset

Statement

Sediment samples were collected from the streambed in June 2015 using an AMS slide hammer with a 5 cm diameter and a trowel to collect sediment from between 0 and 10 cm depth. Sediment was collected from two sites and five pseudo-replicates were collected from each site, and subsequently homogenised and sieved (2 mm) within 36 hours of collection, then stored cold until the beginning of the experiments. Sediment was incubated in three separate incubation experiments to determine rates of FDA hydrolysis, extracellular phenol oxidase activity and potential denitrification. FDA hydrolysis was determined by mixing air-dried sediment with 1 M tris-hydroxymethyl-aminomethane (THAM) buffer and 0.5 ml of FDA substrate, closing the flask and incubating at 37 °C for 3 hours. 2 ml of acetone was then added to the sediment to prevent further reaction. The samples were filtered and fluorescein concentration was determined using a spectrophotometer (Varian Cary UV-Vis). The concentration of fluorescein in the control was subtracted from that in the sample, and then normalised by the mass of soil used for that sample, and the number of hours of the incubation. Extracellular phenol oxidase activity was determined by mixing dried sediment with deionised water, and then adding 10 mM dihydroxy-phenylalanine. The samples were then shaken at 25 °C for 30 minutes, before centrifuging to terminate the reaction. The solution was then filtered and dopachrome concentration was measured using a spectrophotometer (Varian Cary UV-Vis). The potential denitrification was determined by adding stock solution to field-moist sediment in dark bottles. The stock solution used was ultrapure water for control experiments, nitrate solution for nitrate-spiked experiments, glucose solution for carbon-spiked experiments and nitrate+glucose solution for nitrate-carbon-spiked solutions. Sample bottles were made anoxic, acetylene gas was added to prevent the conversion of N2O to N2, and samples were shaken during incubation at 22 °C. Gas samples were taken from the headspace at zero, three and six hours. N2O concentrations in the gas samples were determined on an Agilent 7890A gas chromatograph – micro electron detector. Concentrations were converted to production per mass of sediment per hour by normalising the concentration of fluorescein and dopachrome by the mass of sediment used and the length of the incubation period, and calculating the difference in N2O concentration between zero and six hours and normalising by the mass of sediment used and the length of the incubation period. Results from the experiments were entered into an Excel spreadsheet and exported as a comma separated value file (.csv) for ingestion into the EIDC.

Metadata

File identifier

500193f7-2653-4696-8224-276a734ed6ab XML

Metadata language

English

Character set

8859 Part 1

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2025-11-13T16:16:24

Metadata standard name

UK GEMINI

Metadata standard version

2.3

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Point of contact