53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2021-06-30 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Harpagifer antarcticus and Lipophrys pholis protein metabolism 2021-06-30 creation 2021-06-30 revision 2021-06-30 publication 2021-06-30 released 1.0 https://doi.org/10.5285/53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 doi GB/NERC/BAS/PDC/01516 https://data.bas.ac.uk/ Please cite this item as: Fraser, K. (2021). Harpagifer antarcticus and Lipophrys pholis protein metabolism (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 These data were generated in a comparative study of protein metabolism (protein synthesis, protein growth and protein degradation) in the Antarctic plunderfish, Harpagifer antarcticus and the Northern European blenny, Lipophrys pholis. The study carried out an examination of protein metabolism in these species at a range over overlapping temperatures covering the environmental range of the species. Protein synthesis was measured using the flooding dose methodology in animals held at the British Antarctic Survey in Cambridge, UK. The experimental work was carried out by Andrew Bowgen and Keiron Fraser. The aim of the study was to examine the effect of ambient habitat temperature on protein metabolism in two ecologically similar, but phylogenetically distant fish species, including one that only inhabited polar latitudes. Andrew Bowgen was funded by a NERC PhD studentship and the study was completed as part of the British Antarctic Survey, Biodiversity, Function, Limits and Adaptations from Molecules to Ecosystems (BIOFLAME) project, part of the NERC funded Biological Responses to Extreme Antarctic Conditions and Hyperextremes (BIOREACH) programme. No credit. completed Fraser, Keiron University of Plymouth Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Adaptation EARTH SCIENCE > Oceans > Marine Biology > Fish EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Protein metabolism RNA fish growth polar fishes protein synthesis theme Antarctic Peninsula, Adelaide Island, Rothera Point Antarctica Weymouth United Kingdom place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions Data is released under Open Government Licence V3.0: otherRestrictions No restrictions apply. url url crossReference doi doi crossReference url url crossReference doi doi crossReference doi doi crossReference textTable eng utf8 biota environment oceans -68.125 -68.125 -67.56861 -67.56861 2004-01-01 2004-12-31 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv bytes 11264 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 WWW:LINK-1.0-http--link Get Data Download data download bytes 11264 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Collection and husbandry of fish Harpagifer antarcticus were collected by hand from sublittoral sites at Rothera Point, Adelaide Island, Antarctic Peninsula (67.56861 S, 68.125 W) by SCUBA divers and held in a through-flow aquarium until return to the UK in a refrigerated transport system. In the UK, fish were maintained in a recirculating flow aquarium, under an automatic 12h light : dark regime (water temperature 0°C ± 1.0°C, salinity 34 - 36 PSU). The fish were fed twice weekly to satiation on shelled krill (Euphausia superba). Lipophrys pholis were collected subtidally in Weymouth (UK) by a commercial supplier and fed on chopped white fish (New Zealand Hoki, Macruronus novaezelandiae) as they refused krill. The mean water temperature of the L. pholis stock tank was 14°C ± 1.0°C. With the exception of temperature and diet, husbandry conditions for both species were identical. Temperature acclimation Experimental animals were weighed in water (± 0.1g) and digitally photographed to allow individual identification. The fish were placed in pairs, in jacketed aquaria (18.8 x 20.3 x 22.0cm) containing clean, aerated seawater at their stock tank temperature. Water temperatures were maintained (± 0.1°C) using a thermocirculator (Grant Instruments, Cambridge, UK). Once daily the aquaria were divided in half using a perspex insert, thereby allowing fish to be fed individually to satiation and their food consumption measured (± 0.1g). Adjustment of aquaria to the required experimental water temperatures (H. antarcticus, -1, 1 and 3 °C, L. pholis, 3, 8, 13 and 18 °C) was carried out at a rate of 0.1°C hour-1 (H. antarcticus) or 0.5°C hour-1 (L. pholis), up to a maximum of 0.8°C day-1 (H. antarcticus) or 4°C day-1 (L. pholis). Fish were acclimated to the experimental temperature for 28 days prior to the measurement of protein synthesis, as Antarctic fish can take 3-4 weeks to acclimate to 4 °C and are the slower of the two species to acclimate. The fish were weighed on the first and penultimate day of the acclimation. Specific growth rates (SGR) were calculated for each fish. equation (1) SGR = (ln(W2)-ln(W1)/t) x 100 where SGR is expressed as % body mass. day-1, W1 and W2 represent the mass at the start and end of the acclimatization period respectively and t is the time in days between mass measurements. Protein synthesis measurement Whole animal protein synthesis rates were measured using a modification of the flooding dose method. Groups of fish were injected in the peritoneum with a flooding dose of unlabelled and 3H labelled phenylalanine (10microl.g-1 fish wet mass of 135mmol.l-1 L-[2,6-3H] phenylalanine at 3.6MBq.ml-1 (GE Healthcare, Little Chalfont, UK)). Fish were killed and frozen in liquid nitrogen after 1, 2 and 4h to allow validation of the flooding dose time-course (H. antarcticus, 3°C; L. pholis, 3 and 18°C). In other experimental treatments fish were killed after 2h, H. antarcticus, -1 and 1°C; L. pholis, 8 and 13°C. Baseline, pre-injection phenylalanine concentrations were measured in both species (n=10) to allow the calculation of phenylalanine flooding levels. All samples were frozen in liquid nitrogen and stored at -80°C prior to analysis. Sample analysis Samples were analysed following Fraser et al., (2002). The frozen fish were homogenized in a known volume of ice-cold, 0.2M perchloric acid (PCA). The homogenate was mixed thoroughly and a 2ml aliquot removed for analysis. The aliquot was centrifuged (Eppendorf 5810R, swing bucket rotor, 3980 x g, 10 min, 4°C) to separate the protein precipitate from the intracellular free-pool. The supernatant, was decanted and the NaOH soluble protein in the pellet measured using bovine serum albumin (Sigma-Aldrich, Poole, UK) as the standard. Subsequently, the pellet was ...(34) Data collection: Thermocirculator - Grant Instruments Cambridge Centrifuge - Eppendorf 5810R, swing bucket rotor Rotary evaporator - Buchi Scintillation counter - Wallac 1409 LSC Data quality: Data did not require cleaning and no data was lost. asNeeded completed