5fe25b28-f10b-467b-8812-0b1ee6fd7491 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:22:33 UK GEMINI 2.3 OSGB 1936 / British National Grid Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD) 2024-06-12 publication https://catalogue.ceh.ac.uk/id/5fe25b28-f10b-467b-8812-0b1ee6fd7491 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491 doi: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491 This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided. The work was supported by the Natural Environment Research Council (Grant NE/X011607/1). Full details about this dataset can be found at https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491 Hill, M. Manchester Metropolitan University m.hill@mmu.ac.uk author Jenkinson, L.A. Manchester Metropolitan University lauren.a.jenkinson@stu.mmu.ac.uk author Dean, A.P. Manchester Metropolitan University andrew.dean@mmu.ac.uk https://orcid.org/0000-0001-6893-5118 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Redfern, J. Manchester Metropolitan University j.redfern@mmu.ac.uk https://orcid.org/0000-0003-0958-683X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Shaw, K.J. Manchester Metropolitan University k.shaw@mmu.ac.uk https://orcid.org/0000-0001-9241-4195 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Shaw, K. Manchester Metropolitan University k.shaw@mmu.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Manchester Metropolitan University EMAIL NOT PROVIDED owner Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Environmental risk Cheshire Meres Water chemistry Cyanobacteria Algal blooms Microfluidics Public engagement Lab on a chip otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491 textTable 1000 English utf8 environment 2022-06-20 2022-10-12 -2.403 -2.35 53.307 53.361 Comma-separated values (CSV) jpeg NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/5fe25b28-f10b-467b-8812-0b1ee6fd7491 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/5fe25b28-f10b-467b-8812-0b1ee6fd7491.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls. For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP. The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.