60b777b4-0bd6-48c3-a301-c700854fbfa1 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2024-03-11 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Acclimation and acclimatisation of marine ectotherms collected at Rothera Research Station and Scott Base in Antarctica between 2004 and 2015 2024-03-11 creation 2024-03-11 revision 2024-03-11 publication 2024-03-11 released 1.0 https://doi.org/10.5285/60b777b4-0bd6-48c3-a301-c700854fbfa1 doi GB/NERC/BAS/PDC/01825 https://data.bas.ac.uk/ Please cite this item as: Morley, S.A., Bates, A.E., Clark, M.S., Fitzcharles, E., Smith, R., Stainthorp, R.E., & Peck, L.S. (2024). Acclimation and acclimatisation of marine ectotherms collected at Rothera Research Station and Scott Base in Antarctica between 2004 and 2015 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/60b777b4-0bd6-48c3-a301-c700854fbfa1 This data assesses the ability of 8 species, from 7 classes representing a range of functional groups, to survive, for 100 to 303 days, at temperatures 0 to 4 degrees Celsius above previously calculated long-term temperature limits. Survivors were then tested for acclimation responses to acute warming. Acclimatisation in the field was tested in the seastar Odontaster validus collected in different years, seasons and locations within Antarctica. Finally, we tested the importance of oxygen limitation in controlling survival duration by incubating 7 species under normoxia (20%) and mild hyperoxia (30%). This study was funded by Natural Environment Research Council core funding to the British Antarctic Survey and Spitfire DTP funding to R.E.S. No credit. completed Morley, Simon A British Antarctic Survey Researcher author Bates, Amanda E University of Victoria Researcher author Clark, Melody S British Antarctic Survey Researcher author Fitzcharles, Elaine British Antarctic Survey Researcher author Smith, Rebecca British Antarctic Survey Researcher author Stainthorp, Rose E National Oceanography Centre Researcher author Peck, Lloyd S British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Adaptation EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctic Southern Ocean acclimation acclimatisation climate change physiology resilience warming theme Ryder Bay, Rothera Research Station Antarctica Ross Island, Scott Base Antarctica place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions None. url url crossReference textTable eng utf8 biota environment oceans 166.76833 166.76833 -77.84916 -77.84916 2004-01-01 2015-12-31 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv bytes 64512 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=60b777b4-0bd6-48c3-a301-c700854fbfa1 WWW:LINK-1.0-http--link Get Data Download data download bytes 64512 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=60b777b4-0bd6-48c3-a301-c700854fbfa1 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Experiments were conducted between 2006 and 2015 with individuals hand collected by SCUBA divers in the austral summer, from 6 - 15m depth, near Rothera Research Station, Adelaide Island (67° 34' 2'' S, 68° 08' 0'' W). Common marine ectotherms were selected from different phyla to represent a range of functional groups, for which aquarium husbandry is well established and long term temperature limits have been estimated under normoxia. All animals remained submerged throughout the transfer from the sea to the flow-through aquarium system at the station. To control for size-dependent effects on survival we selected individuals of a similar size within each species group at the start of the experiment, and only studied fully reproductive adults. The 3.0 °C temperature-oxygen experiment was conducted in flow through aquaria at Rothera Research Station. In Rothera, the tanks had a constant exchange of seawater that was balanced to allow temperature and oxygen treatments to be maintained while preventing any build-up of metabolic waste. In all experiments, seawater chemistry was monitored every 2-3 days using Nutrafin aquarium test kits. Ammonia, nitrite and nitrates were maintained well below 0.4, 0.2 and 5 mg L-1 to prevent toxicity from metabolic by-products. A small piece of one tentacle was removed from each anemone and preserved in 96% ethanol. DNA was extracted from each tentacle using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions. The cytochrome oxidase subunit I gene (COI) mitochondrial region was amplified using 1-2µl extracted DNA and MyTaq DNA polymerase mix (30µl reactions; Bioline UK (now Meridien Biosceince), with 10nmol each of the universal COI primers for invertebrates (LCO 1490 5'-GGTCAACAAATCATAAAGATATTGG-3'; HCO 2198 5'''''''-TAAACTTCAGGGTGACCAAAAAATCA-3') (Folmer et al. 1994). PCR conditions were: 94 °C for 5 minutes, 5 cycles of 94 °C for 1 min, 45 °C for 1.5 min, 72 °C for 1.5 min, followed by 30 cycles of 94 °C for 1 min, 50 °C for 1 min, 72 °C for 1 min and a final elongation stage of 5 min at 72 °C. The COI fragments were bi-directionally sequenced by Source Bioscience (Cambridge, UK). The species identity of each individual was analysed using Blast sequence similarity searching of INSDC (International Nucleotide Sequence Database Collaboration) (https://www.insdc.org/). Temperatures were raised at the same rate in all experiments (0.3 ± 0.1 °C d-1) until the incubation temperature was reached and subsequently monitored daily. Animals were held at temperatures of 6.0 and 8.0 °C, temperatures that were 0 to 4 °C above the long-term limits calculated from experiments with different rates of warming. Control animals were kept at 0.0 °C in the main holding aquarium. Acute thermal limits: Individuals that survived beyond the duration of incubations at both 6.0 and 8.0 °C were then tested to see if acute thermal limits were elevated due to acclimation to these elevated incubation temperatures. These data were compared with acute thermal limits conducted on control individuals that had been kept in the aquarium at 0.0 °C. For the acute temperature ramping trials individuals were transferred to plastic jacketed tanks (Engineering Design and Plastics Ltd), whose jackets were filled with 25% v/v ethanol in water solution, that was heated or cooled by LTD20G thermocirculators (Grant Instruments). Temperatures were increased at 1.0 ± 0.1 °C d-1 until the last animal was no longer responding to the stimuli detailed above. Acclimatisation in field animals was tested for in only one species, the common starfish O. validus. To test for field acclimatisation the assessment of CTmax of freshly collected O. validus was repeated between 2006 and 2015 in both summer and winter. There...(8) Data quality: All data have been quality checked by the data creators. asNeeded completed