Date (Creation)

2017-08-08

Date (Revision)

2017-08-08

Date (Publication)

2017-08-08

Date (released)

2017-08-08

Edition

1

Unique resource identifier

https://doi.org/10.5285/6386ed1d-fe95-4c8e-b471-74757d831bd1

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/00989

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/H014446/1

Codespace

award

Unique resource identifier

NE/H014802/1

Codespace

award

Other citation details

Please cite this item as: None

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Convey, Pete		Author
University of Sheffield	Hodson, Andy		Author
British Antarctic Survey	Pearce, David		Author
University of Bristol	Sabacka, Marie		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Ecological Dynamics > Primary Production
• EARTH SCIENCE > Biosphere > Ecological Dynamics > Secondary Production
• EARTH SCIENCE > Cryosphere > Glaciers/Ice Sheets > Glaciers
• EARTH SCIENCE > Cryosphere > Glaciers/Ice Sheets > Ice Sheets
• EARTH SCIENCE > Biosphere > Microbiota

Theme

• Bacterial production

• Glacier

• Primary production

• Signy Island

• Snow

• Superimposed ice

Place

• Gourlay Snowfield and Tuva Glacier, Signy Island Antarctica

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes
• Elevation

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

Requests for data can be made via the UK Polar Data Centre (UK PDC) at BAS.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Larger work citation

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Climatology, meteorology, atmosphere
• Environment

Begin date

2012-12-01

End date

2013-03-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

The selected pits formed a diagonal in the direction of the melt. Snow samples of top and middle layer were collected from these three pits and melted overnight. 150 ml of melted snow was added to B.O.D. borosilicate bottles to which 7.7 uCi (284.9 kBq) of 14C bicarbonate was added. 5 replicates for each sample: 3 clear bottles (live), 1 bottle dark bottle that did not allow any light penetration (dark) and a bottle to which 5% of filtered buffered parafolmaldehyde was added (kill). Microorganisms in the 'kill' bottle were killed with formaldehyde prior to the addition of the 14C isotope. Bottles were in clear plastic trays filled with snow and added onto the surface of the pit (TOP) or buried at the bottom of the pit (MID). Samples were incubated for 24 hours. Low light condition was ensured when handling the samples by using a shelter to cover from sunlight. Isotope was added just prior to experiment on site. The trays with vials were attached to snow stakes with ropes to ensure no movement. The experiment was conducted at the beginning of the season (December) and at the end of the season (March).

At the end of the incubation, samples were put into a dark box and immediately transferred to the Signy laboratory (up to 1 hour). Afterwards, samples were filtered in darkened lab under low pressure (<7 psi) onto 25 mm GFF filters (combusted at 450°C for 5 hours) that were carefully positioned at the bottom of clean glass scintillation vials. 0.5 ml of 3N HCl was added to each vial and samples were dried in a fume hood at 60°C for up to 8 hours. Scintillation vials containing dried filters were transported to Sheffield University at room temperature. In the laboratory, 10 ml of scintillation cocktail was added to each sample and run on Scintillation counter. Disintegration per minute (dpm) was calculated on scintillation counter and recalculated to uptake of carbon in .micro grams (ug) of Carbon per litre (l) per day (d).

Bacterial production uptake was measured using leucine uptake incubation following modified version of method described by Smith et al. (1992). Briefly: snow and ice were collected into autoclaved and acid-washed 1 L amber HDPE bottles. Samples were melted overnight at +4°C. 1.5 ml of snow or ice melted was added to a 2 mL plastic centrifuge tubes (autoclaved). Five replicates per sample: three live controls and two kill controls. Each sample contained 4.4 µCi (162.8 kBq) of tritiated leucine, wiith final concentration of 20nM leucine per sample. Kill controls were killed with 100 µl of 100% cold trichloroacetic acid (TCA) prior to the addition of 3H isotope (final concentration %). Tray with vials was covered in aluminium foil in order to prevent light penetration and was placed at the same site of sampling and incubated for 24 hours. The experiment was stopped by addition of 100 µl of 100% TCA. Samples were kept at +4 C and in the dark prior and during transportation to the laboratory in Sheffield.

1.5 mL of scintillation cocktail (Monophase®) was added to each sample. Disintegration per minute (dpm) was calculated on scintillation counter and recalculated to uptake of carbon in .micro grams (ug) of Carbon per litre (l) per day (d).

Data collection:

Instrumentation used:

Scintillation counter

Metadata

File identifier

6386ed1d-fe95-4c8e-b471-74757d831bd1 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2017-08-08

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact