64607f86-0228-4a03-9d87-6e36435028a1 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:19:38 UK GEMINI 2.3 OSGB 1936 / British National Grid Seed traits, seed viability and germination rates of seeds collected from 12 natural Juniper (Juniperus communis) populations in the UK, 2023-2025 2025-09-25 publication https://catalogue.ceh.ac.uk/id/64607f86-0228-4a03-9d87-6e36435028a1 10.5285/64607f86-0228-4a03-9d87-6e36435028a1 doi: Baker, J., Cottrell, J., Ennos, R., Perry, A., Cavers, S. (2025). Seed traits, seed viability and germination rates of seeds collected from 12 natural Juniper (Juniperus communis) populations in the UK, 2023-2025. NERC EDS Environmental Information Data Centre 10.5285/64607f86-0228-4a03-9d87-6e36435028a1 These data describe the rates of filled, viable and germinating seeds from over 60 maternal juniper trees from across the UK. 12 populations from the Scottish Highlands to Southern English Downs were sampled in Fall 2023. Four different methods of seed viability testing were applied, and germination was monitored for over a year in response to nine different combinations of pretreatments and stratifications. Reproducible analyses and visualizations from an associated study are provided with an R project. This trial was conducted to determine the optimal treatment for juniper stratification and to quantify the variability in seedlot quality/viability among populations. Full details about this dataset can be found at https://doi.org/10.5285/64607f86-0228-4a03-9d87-6e36435028a1 James Baker UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0009-0003-0514-4644 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Baker, J. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0009-0003-0514-4644 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Cottrell, J. Forest Research joan.cottrell@ForestResearch.gov.uk author Ennos, R. University of Edinburgh rennos@ed.ac.uk author Perry, A. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Cavers, S. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Species Distribution theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication germination stratification seed (biology) plant population conservation conservation of species forest conservation forest biodiversity GEMET - Concepts, version 4.1.3 2019-01-28 creation Evolutionary ecology Common Juniper otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Baker, J., Cottrell, J., Ennos, R., Perry, A., Cavers, S. (2025). Seed traits, seed viability and germination rates of seeds collected from 12 natural Juniper (Juniperus communis) populations in the UK, 2023-2025. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/64607f86-0228-4a03-9d87-6e36435028a1 textTable English utf8 biota environment 2023-01-01 2025-12-31 -8.702 1.768 49.864 59.445 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/64607f86-0228-4a03-9d87-6e36435028a1 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/64607f86-0228-4a03-9d87-6e36435028a1.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Berries were collected from populations in fall 2023, weighed, and inspected for parasitism. Seeds were extracted with a food processor; filled and empty seeds were separated using the float test, then surface sanitized and dried. Dried seeds were weighed and stored at 4 degrees C until used. Seeds were then treated with one of two different pretreatments, consisting of soaking them in water or a 1% Citric Acid solution for 48 hours. A Control group was also included that was not pretreated. Next, seeds were subject to one of 4 different thermal stratification treatments: A warm stratification followed by a cold stratification; just a warm stratification; just a cold stratification; and a control without a stratification treatment. Germination was then monitored for over a year, and the differences among seedlots, populations and regions of the UK were quantified. Additional seedlot quality and seed viability data were also collected for all 60 seedlots included in the germination trial. The field cut test (the proportion of seeds that are visibly filled with healthy embryonic tissue when bisected), the estimated numbers of filled and empty seeds from the float test, tetrazolium chloride (TZ) staining and X-ray viability are each applied to each seedlot. These results are then compared and contrasted to evaluate their relative accuracy and precision.