65359151-158c-47d1-8c04-a59bc3a28f53 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2020-10-07 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Relative abundances of DNA and RNA of a previously undescribed Helotiales species in soils from Signy Island and Leonie Island, along with edaphic factors 2020-10-07 creation 2020-10-07 revision 2020-10-07 publication 2020-10-07 released 1.0 https://doi.org/10.5285/65359151-158c-47d1-8c04-a59bc3a28f53 doi GB/NERC/BAS/PDC/01403 https://data.bas.ac.uk/ NE/H014098/1 award NE/H014772/1 award NE/H01408X/1 award Please cite this item as: Robinson, C., Cox, F., Garnett, M., Horrocks, C., Dungait, J., & Newsham, K. (2020). Relative abundances of DNA and RNA of a previously undescribed Helotiales species in soils from Signy Island and Leonie Island, along with edaphic factors (Version 1.0) [Data set]. UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation. https://doi.org/10.5285/65359151-158c-47d1-8c04-a59bc3a28f53 The dataset consists of the relative abundances of the DNA and RNA of a fungus in soil samples from Signy and Leonie Islands, along with physico-chemical parameters (moisture concentration, pH value, total carbon and nitrogen concentrations, delta-carbon-13 content, carbon-14 enrichment, and mean carbon residence time). Funding was provided by NERC grants NE/H014098/1 and NE/H014772/1 and NE/H01408X/1 No credit. completed Robinson, Clare H. University of Manchester Researcher author Cox, Filipa University of Manchester Researcher author Garnett, Mark NERC Researcher author Horrocks, Claire Rothamsted Research Researcher author Dungait, Jennifer Rothamsted Research Researcher author Newsham, Kevin British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Fungi theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctica Carbon-13 Carbon-14 Helotiales soil age theme Polynesia Point, Signy Island Antarctica Walton Terraces, Leonie Island Antarctica place Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ url url crossReference textTable eng utf8 biota -45.5849 -68.3561 -67.5984 -60.7107 2011-11-01 2015-12-31 35.0 60.0 urn:ogc:def:crs:EPSG::5714 MSL height Not specific to any location or epoch. Hydrography. It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/csv bytes 14336 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=65359151-158c-47d1-8c04-a59bc3a28f53 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Soils used in the analyses were sampled in October and November 2011 from active layers overlaying permafrost in six pits (0.40 x 0.40 m) dug under turves of Antarctic Hairgrass (Deschampsia antarctica). Three pits were dug at Polynesia Point on Signy Island (60.7107 deg S, 45.5849 deg W; altitude 34-45 m a.s.l.) in the South Orkney Islands and three were dug on Walton Terraces, on the north-eastern side of Leonie Island (67.5984 deg S, 68.3561 deg W; altitude 50-60 m a.s.l.) in the southern maritime Antarctic. The three pits at each island were separated by a mean distance of 311 m. Soils were sampled by hammering five sterile tubes (50 ml capacity) horizontally into the vertical faces of each pit at depths of 2, 4 and 8 cm, generating a total of 90 soil samples. Further details of sampling are provided in Cox et al. (2016; 2019) and Newsham et al. (2018). Soil was frozen at -20 deg C or -80 deg C within a few hours of sampling and was transported back to the UK at these temperatures, where it was thawed prior to analyses. Soil pH, moisture and C and N concentrations were measured in bulked soils from each of the three depths in the three pits dug at each island, generating 18 samples in total. Soil pH was measured by adding approximately the same volume of deionised water to each soil sample to generate slurries and recording pH with a glass electrode (pH 21, Hanna Instruments, Leighton Buzzard, UK). Soil moisture was measured gravimetrically after drying c. 1 g of fresh soil at 105 deg C for 17 h. Sub-samples (c. 2.5 mg) of air-dried soil were weighed into foil capsules and were analysed for total C and N. Carbon-14 and Carbon-13 determinations were made on the five replicate soils sampled from depths of 2, 4 and 8 cm in one of the three pits dug at each island, generating 30 samples in total. The soils were sent to the NERC (now NEIF) Radiocarbon Laboratory, where the samples were converted to carbon dioxide using either bomb combustion in a high-pressure oxygen atmosphere, or an elemental combustion system. Sample carbon dioxide was cryogenically purified under vacuum and split into aliquots. The delta carbon-13 (relative to the Vienna PDB standard) was determined on one aliquot of sample carbon dioxide by isotope ratio mass spectrometry. A second aliquot was converted to graphite using Fe: Zn reduction and analysed for carbon-14 content using accelerator mass spectrometry at the Scottish Universities Environmental Research Centre (East Kilbride, UK). Carbon-14 results were normalised to delta carbon-13 = -25 per mille and expressed as %modern and conventional radiocarbon ages (in years BP, where 0 BP = AD1950). Final mean residence time (MRT) of C in soil was determined using the Meathop Model, as described by Horrocks et al. (2020). The model calculates a maximum carbon-14 concentration of 114.33 %Modern (for a MRT of 30 years). This MRT was assigned to all values of >114 % Modern. Note that for sample FC_31, there were two solutions to the model, because of the rising and falling parts of the bomb-carbon-14 curve. Also note that for carbon-14 enrichment values of <97% Modern, a conventional radiocarbon age (CRA) was assigned for mean residence time since these samples showed no evidence of bomb-carbon-14 incorporation (Bol et al., Eur. J. Soil Sci. 50, 41-51, 1999). As described by Cox et al. (2016; 2019), DNA and RNA were extracted simultaneously from soil samples (5 mg) taken from the 50 ml-capacity tubes using RNA PowerSoil Total RNA Isolation and DNA Elution Accessory kits (MoBio Laboratories, Carlsbad, CA, USA). Extracted RNA was treated with a Turbo DNA-free kit (Life technologies, Carlsbad, CA, USA), checked for contaminant DNA using PCR, and reverse transcribed using AccuScript High-Fidelity Reverse Transcriptase (Agilent, Santa Clara, CA, USA) and random nonamers. Internal transcribed spacer (ITS) regions in the extr...(13) Data collection: The following instruments were used in the analyses: 1. Total nitrogen and organic carbon concentrations: Carlo Erba NA1500 elemental analyser, CE Instruments, Wigan, UK 2. pH value: Hanna Instruments pH meter, Leighton Buzzard, UK 3. Elemental combustion: Costech ECS 4010, Italy 4. Isotope ratio mass spectrometry: Delta V, Thermo-Fisher, Germany 5. Bead purification: Beckman Coulter, Inc, Brea, CA, USA 6. Qubit dsDNA HS Assay: Life Technologies, Carlsbad, CA, USA Data quality: Instruments were calibrated according to laboratory practices, with standards being deployed where necessary. No data losses and no data cleaning. asNeeded completed