65c7085a-7bcf-481a-884a-8e1c9cfc395b English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:23:23 UK GEMINI 2.3 Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa 2024-05-08 publication 2024-04-10 creation https://catalogue.ceh.ac.uk/id/65c7085a-7bcf-481a-884a-8e1c9cfc395b 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b doi: Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b The resource contains experimental data collected at the Institute of Ecology and Evolution, University of Edinburgh between Jan-Oct 2023. Sixty (60) lines from the Drosophila Genetic Reference Panel (DGRP) were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed. Total number of activity events were measured for each fly individually using Drosophila Activity Monitor (DAM), measured at 1 min resolution until death. Mortality was recorded as the last detected movement in DAM. Pathogen shedding was measured for each individual fly as the number of viable P. aeruginosa colony-forming units defecated by an infected fly for 24h following oral infection. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b Vale, P. University of Edinburgh pedro.vale@ed.ac.uk https://orcid.org/0000-0003-4558-9202 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Vale, P. University of Edinburgh pedro.vale@ed.ac.uk https://orcid.org/0000-0003-4558-9202 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Kutzer, M. University of Edinburgh mkutzer@exseed.ed.ac.uk https://orcid.org/0000-0002-8696-6978 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Edinburgh EMAIL NOT PROVIDED owner Pseudomonas aeruginosa Wikidata 2012-10-29 creation infection pathogen mortality GEMET - Concepts, version 4.1.3 2019-01-28 creation Animal behaviour Drosophila transmission super-shedding activity heterogeneity otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b English utf8 biota Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/65c7085a-7bcf-481a-884a-8e1c9cfc395b Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/65c7085a-7bcf-481a-884a-8e1c9cfc395b.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Sixty (60) lines from the DGRP were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed individually to infection overnight. Each treatment was replicated 5-10 times (707 samples in total). The experiment was split among 5 experimental blocks with a variable number of replicates tested in each, as described in the data file. Following infection (see above) pathogen shedding was measured by placing each individual fly inside a sterile Eppendorf and then quantifying the number of viable P. aeruginosa colony-forming units defecated each infected fly for 24h following oral infection. CFU (colony forming unit) plating was carried out on Pseudomonas sp. selective media (PIM) to select for P. aeruginosa CFUs only. Each fly was then transferred to Drosophila Activity Monitor (DAM) and the total number of activity events were measured for each fly individually measured at 1 min resolution until death (2500-~20,000 minutes). Data generated in the DAM included the total number of activity bouts recoded for each minute for the duration of the experiment (2500-~20,000 minutes). The values reported in the resource are the total number of activity bouts recoded per fly until death or until the end of the experiment. Mortality was recorded as the last detected movement in DAM.