65fb253d-2de3-4953-940a-525a1c3614d8 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2024-07-03 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Molecular analysis of Calanus from the Fram Strait 2019 2024-07-03 creation 2024-07-03 revision 2024-07-03 publication 2024-07-03 released 1 65fb253d-2de3-4953-940a-525a1c3614d8 uuid GB/NERC/BAS/PDC/01858 https://data.bas.ac.uk/ NE/P006213/1 award NE/P006353/1 award NE/P006302/1 award Please cite this item as: None Multidisciplinary field campaigns were carried out in the Fram Strait region during late summer 2019 (JR18007, 04/08/2019 to 28/08/2019) aboard RRS James Clark Ross. A motion-compensated Bongo net fitted with 200 um mesh was deployed at all locations to a maximum depth of 200 m or to within 10 m of the seabed.In the Arctic and sub-Arctic sectors adjacent to the Atlantic, three species of Calanus co-occur. However, their respective core distributions align with different water masses, with Calanus hyperboreus being a high-Arctic oceanic species, C. glacialis being associated with Arctic shelf waters, and C. finmarchicus dominating inflowing Atlantic Water.The proportions of these three species in each deployment was established through molecular analysis of the paired ethanol preserved sample. Subsamples of the ethanol sample were analysed using a 16S ribosomal RNA gene barcode (16SAR, 16SB2R primers) following an adapted protocol. Amplified DNA was sequenced using llumina high-throughput sequencing (HTS) platform. Resultant sequences processed through the Qiime pipeline, clustered into Operational Taxonomic Units at 97% homology and taxonomy was assigned using BLASTn (NCBI). Funded by UKRI Natural Environment Research Council (NERC; NE/P006213/1, NE/P006353/1, NE/P006302/1, NE/ P005985/1 amongst others). No credit. completed Parry, Helen Plymouth Marine Laboratory Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Animal Taxonomy > Zooplankton EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Zooplankton theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher 16S ribosomal RNA Calanus bongo net zooplankton theme Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ otherRestrictions No restrictions apply. url url crossReference textTable eng utf8 biota -180.0 180.0 -90.0 90.0 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor bytes 1 https://www.ncbi.nlm.nih.gov/sra/PRJNA838801 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: The 16S rRNA gene amplified using the primer pair 16SAR and 16S2R and sequenced using MiSeq by commercial contract (MR DNA, Texas, USA) Multidisciplinary field campaigns were carried out in the Fram Strait region during late summer 2019 (JR18007, 04/08/2019 to 28/08/2019) aboard RRS James Clark Ross. A motion-compensated Bongo net fitted with 200 um mesh was deployed at all locations to a maximum depth of 200 m or to within 10 m of the seabed.In the Arctic and sub-Arctic sectors adjacent to the Atlantic, three species of Calanus co-occur. However, their respective core distributions align with different water masses, with Calanus hyperboreus being a high-Arctic oceanic species, C. glacialis being associated with Arctic shelf waters, and C. finmarchicus dominating inflowing Atlantic Water.The proportions of these three species in each deployment was established through molecular analysis of the paired ethanol preserved sample. Subsamples of the ethanol sample were analysed using a 16S ribosomal RNA gene barcode (16SAR, 16SB2R primers) following an adapted protocol. Amplified DNA was sequenced using llumina high-throughput sequencing (HTS) platform. Resultant sequences processed through the Qiime pipeline, clustered into Operational Taxonomic Units at 97% homology and taxonomy was assigned using BLASTn (NCBI). Data collection: motion-compensated Bongo net fitted with 200 um mesh llumina high-throughput sequencing (HTS) platform asNeeded completed