6bc69e2a-c771-4538-832e-2f6824b63474 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-21T09:36:54 UK GEMINI 2.3 Functionalisation data of a carbon electrode biosensor for detection of antimicrobial resistance 2022-01-12 publication https://catalogue.ceh.ac.uk/id/6bc69e2a-c771-4538-832e-2f6824b63474 10.5285/6bc69e2a-c771-4538-832e-2f6824b63474 doi: Mitrakos, V., Al Mshelesh, H., Bridle, H., Desmulliez, M. (2022). Functionalisation data of a carbon electrode biosensor for detection of antimicrobial resistance. NERC EDS Environmental Information Data Centre 10.5285/6bc69e2a-c771-4538-832e-2f6824b63474 The dataset consists of results of the electrochemical circle fit of the Nyquist plot generated by NOVA software to measure the Charge Transfer Resistance (RCT). Data demonstrates the curve of real and imaginary resistance against charges transmitted in the media of the carbon electrode biosensor. The change in RCT can detect the presence of the targeted antimicrobial resistance (AMR) DNA. The experiments were conducted by immersing the sensor's electrode in different concentrations of the target AMR DNA to determine the limit of detection. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/6bc69e2a-c771-4538-832e-2f6824b63474 Mitrakos, V. Heriot-Watt University zmitrakos@gmail.com author Al Mshelesh, H. Heriot-Watt University Hk49@hw.ac.uk author Bridle, H. Heriot-Watt University h.l.bridle@hw.ac.uk https://orcid.org/0000-0001-9068-7624 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Desmulliez, M. Heriot Watt University m.desmulliez@hw.ac.uk https://orcid.org/0000-0002-2441-1598 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Al Mshelesh, H. Heriot-Watt University Hk49@hw.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Heriot-Watt University EMAIL NOT PROVIDED owner Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication resistance (biological) GEMET - Concepts, version 4.1.3 2019-01-28 creation Antimicrobial resistance Electrochemical sensor Biosensor waterborne bacteria otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Mitrakos, V., Al Mshelesh, H., Bridle, H., Desmulliez, M. (2022). Functionalisation data of a carbon electrode biosensor for detection of antimicrobial resistance. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/6bc69e2a-c771-4538-832e-2f6824b63474 English utf8 environment 2021-07-15 2021-07-29 JPEG NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/6bc69e2a-c771-4538-832e-2f6824b63474 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/6bc69e2a-c771-4538-832e-2f6824b63474.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The fabrication process involves multi LTCC layers, and screen printing of silver reference electrode (RE), carbon layer of working electrode (WE) and counter electrode (CE), and the dielectric layers. The SPCEs were functionalised with a specific (Peptide nucleic acid) PNA probe, by electrografting through in situ generated diazonium cations. Then creating a 4-carboxyphenyl (AP) film on the electrode surface after the modification of SPCEs electrochemical by reduction of the in situ generated 4-carboxyphenyl diazonium salt, using four cyclic voltammetry (CV) scans from +0.4 to -0.6 V at a scan rate of 100mV/s. The AP film then activated via 60 min incubation in 1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide (EDC) and NHS (N-hydroxysuccinimide) in a MES (2-(Nmorpholino) EthaneSulfonic acid), PH 5.0. After, the electrode surface rinsed in MES buffer, and incubated it in amino-modified PNA probe dispersed in droplet of PBS buffer for 60 min at room temperature. Formation of a blocking film by droplet deposition to the electrode of 1% v/v ethanolamine/PBS buffer and incubated for 30 min in room temperature. Finaly, 52 min incubation with complimentary DNA target and electrochemical impedance spectroscopy (EIS) measurements by (Metrohm-Autolab, PGSTAT204/FRA32M), (dRCT 100kHz to 0.3Hz) in a 1mM KCl + 2mM Ferri/Ferrocyanide solution. DNA strand hybridizing to probs lead to an accumulation of negative charge on the electrode surface causing an increase in the charge transfer resistance (RCT).