6c027bf9-b344-4178-b8b3-d78cf39f017f English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:16:24 UK GEMINI 2.3 OSGB 1936 / British National Grid Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats 2016-04-25 publication 2013-08-31 creation https://catalogue.ceh.ac.uk/id/6c027bf9-b344-4178-b8b3-d78cf39f017f 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f doi: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f The dataset details surface sediment colloidal carbohydrate concentrations across six intertidal sites in the winter and summer of 2013. Colloidal carbohydrate concentrations in surface sediments (top 2mm) provide a quantitative measure of the extracellular polymeric substances (EPS) secreted by organisms that form the microphytobenthos (MPB) community. Three of the sites were in Morecambe Bay, North West England and three of the sites were in Essex, South East England, each of these sites consisted of a saltmarsh area and adjacent mudflat area, twenty two sampling quadrats were placed on each area. Five replicate sediment samples were taken at each quadrat and were analysed using the Dubois Phenol-Sulphuric Assay which involves spectrophotometer analysis of absorptions to determine colloidal carbohydrate concentrations. Values for colloidal carbohydrate concentrations are expressed as glucose equivalents in micrograms per gram of sediment. This data was collected as part of Coastal Biodiversity and Ecosystem Service Sustainability (CBESS): NE/J015644/1. The project was funded with support from the Biodiversity and Ecosystem Service Sustainability (BESS) programme. BESS is a six-year programme (2011-2017) funded by the UK Natural Environment Research Council (NERC) and the Biotechnology and Biological Sciences Research Council (BBSRC) as part of the UK's Living with Environmental Change (LWEC) programme. Full details about this dataset can be found at https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f Maunder, J. University of St Andrews jm280@st-andrews.ac.uk pointOfContact Maunder, J. University of St Andrews jm280@st-andrews.ac.uk author Paterson, D.M. University of St Andrews dp1@st-andrews.ac.uk author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher University of St Andrews EMAIL NOT PROVIDED owner notPlanned Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication salt marsh sediment GEMET - Concepts, version 4.1.3 2019-01-28 creation Morecambe Bay Essex GeoNames 2006-01-01 creation BESS Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) sediment colloidal carbohydrates surface sediment mudflat contact core microphytobenthos MPB Dubois assay otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © University of St Andrews otherRestrictions If you reuse this data, you should cite: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f textTable English utf8 oceans 2013-01-01 2013-08-31 -3 1.005 51.687 54.25 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/6c027bf9-b344-4178-b8b3-d78cf39f017f Download the data Download a copy of this dataset download https://data-package.ceh.ac.uk/sd/6c027bf9-b344-4178-b8b3-d78cf39f017f.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Sediment colloidal carbohydrate concentration was determined through the collection of contact cores. Contact cores use liquid nitrogen to freeze the top 2mm of the sediment surface, excess unfrozen sediment is removed and the sample core is stored below -80 degree centigrade. The sample is weighed to determine wet weight and freeze dried to remove all water. Colloidal carbohydrate concentration is determined using the Dubois Phenol-Sulphuric assay. A sediment sub-sample (50-500mg, depending on site) is taken from dried contact core sediment and known volume of distilled water is added. Sample is vortexed and centrifuged then a set volume of supernatant is removed. Concentrated sulphuric acid and 5 percent phenol are added to the supernatant sample ensuring the ratio of sample: phenol: acid is kept at 1:1:5 by volume. Allow 35mins for colour to develop. Decant the samples into cuvettes and read the absorbance at 486.5nm on Thermo Biomate 5 spectrophotometer. Use figures from regression analysis of glucose standards to calculate colloidal carbohydrate concentration expressed as glucose equivalents in micrograms per gram (µg g-1). Note: zeroes indicate colloidal carbohydrate concentration below detectable level. Twenty two 1 x 1 m quadrats were randomly allocated to each mudflat and saltmarsh site using R (R Development Core Team, 2014) to specify four different spatial scales (A = 1 quadrat only, B = 3 quadrats at 1 m to 10 m apart, C = 6 quadrats at 10 m to 100 m apart, D = 12 quadrats at 100 m to 1000 m or site maximum).