BGS Hosted Metadata
Greenhouse gas exchange, temperatures, bacterial and fungal abundances and the relative abundances of the 40 most frequent bacterial taxa in a soil warming and irrigation experiment on Svalbard
Data on CO2 and CH4 exchange rates between soil and atmosphere, soil temperatures, bacterial 16S ribosomal RNA genes, fungal internal transcribed spacer 2 (ITS2) copies and the relative abundances of the 40 most abundant bacterial taxa in the 48 plots of a soil warming and irrigation experiment on Svalbard in the High Arctic.
On 2014-09-10, a soil warming and irrigation experiment was set up at Kongsfjordneset on the Brogger Peninsula, Svalbard. Warming was applied continuously with open top chambers and the irrigation treatment was applied in mid-late June and late August each year. Greenhouse gas exchange between the soil and atmosphere was measured on 2018-08-23 and 2018-08-26. At this time, soil samples were taken for DNA analyses and the amount of bacterial and fungal DNA present in soil was measured. The 40 most frequent bacterial operational taxonomic units were also determined.
This project was funded by UK Natural Environment Research Council (core funding to the British Antarctic Survey), the Danish National Research Foundation (CENPERM DNRF100) and Seoul National University.
Simple
- Date (Creation)
- 2022-09-14
- Date (Revision)
- 2022-09-14
- Date (Publication)
- 2022-09-14
- Date (released)
- 2022-09-14
- Edition
-
1
- Unique resource identifier
- https://doi.org/10.5285/71dfd312-a493-42fc-822a-29f3dca66dc0
- Codespace
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doi
- Unique resource identifier
- GB/NERC/BAS/PDC/01668
- Codespace
- Other citation details
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Please cite this item as: https://doi.org/jd4g
- Credit
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No credit.
- Status
- Completed
- Point of contact
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Organisation name Individual name Electronic mail address Role British Antarctic Survey Newsham, Kevin
Author Center for Permafrost
Danielsen, Birgitte
Author British Antarctic Survey Biersma, Elisabeth
Author Center for Permafrost
Elberling, Bo
Author British Antarctic Survey Hillyard, Guy
Author Seoul National University
Kumari, Priyanka
Author Center for Permafrost and Department of Biology
Prieme, Anders
Author Seoul National University
Woo, Cheolwoon
Author Seoul National University
Yamamoto, Naomichi
Author NERC EDS UK Polar Data Centre Point of contact
- Maintenance and update frequency
- As needed
- Maintenance note
- Completed
- Theme
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Svalbard
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carbon dioxide
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climate change
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cryoturbation
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frost boils
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greenhouse gases
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methane
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non-sorted circles
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soil bacteria
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soil fungi
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- Place
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Kongsfjordneset, Brøgger Peninsula Svalbard
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GEMET - INSPIRE themes, version 1.0
- Access constraints
- Other restrictions
- Other constraints
- no limitations to public access
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- License
- Other constraints
- This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/
- Use constraints
- Other restrictions
- Other constraints
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No restrictions apply.
- Unique resource identifier
- doi
- Codespace
-
doi
- Association Type
- Cross reference
- Unique resource identifier
- url
- Codespace
-
url
- Association Type
- Cross reference
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Environment
)
- Begin date
- 2014-09-19
- End date
- 2018-08-27
- Supplemental Information
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It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.
- Title
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European Petroleum Survey Group (EPSG) Geodetic Parameter Registry
- Date (Publication)
- 2008-11-12
- Cited responsible party
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Organisation name Individual name Electronic mail address Role European Petroleum Survey Group
Publisher
- Unique resource identifier
- urn:ogc:def:crs:EPSG::3031
- Version
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6.18.3
Distributor
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Distributor
- Units of distribution
-
bytes
- Transfer size
- 1
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=71dfd312-a493-42fc-822a-29f3dca66dc0 Get Data
- Hierarchy level
- Dataset
- Statement
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Methodology:
On 2014-09-10, a soil warming and irrigation experiment was set up at Kongsfjordneset on the Brogger Peninsula, Svalbard. It consists of 48 plots centred on individual frost boils treated with a factorial combination of warming with open top chambers and irrigation. The experimental design results in four OTC-irrigation treatments, each replicated 12 times across three blocks. The OTCs have a basal diameter of 1.04 m. The irrigation treatment consisted of applying 1 L of deionised water to 24 of the frost boils in mid-late June and late August each year, simulating c. 20 mm rainfall events.
Frost boil temperatures were monitored by burying Tinytag Plus 2 loggers in soil in four chambered and four unchambered plots. The loggers recorded temperatures at a depth of 30-35 mm between 2014-09-10 and 2018-08-27. They were replaced yearly with newly calibrated units. Measurements recorded between September 2017 and August 2018 by two loggers that had become exposed at the soil surface in summer 2018 were deleted from the dataset.
Gas exchange between the soil and atmosphere was measured twice in each of the 48 frost boils, on 2018-08-23 and 2018-08-26, using a closed loop system and a Piccaro Gas Analyzer. The analyzer was attached to a transparent polycarbonate chamber equipped with fans for air circulation seated on stainless steel frames that had been hammered into the soil in each boil on 2018-06-30. Gutters filled with water around each frame ensured an airtight seal. The chamber was covered with dark cloth to eliminate photosynthetically active radiation during the measurements. CO2 and CH4 exchange was measured over a period of 5 min. The fluxes of both gases were calculated by fitting 2nd order polynomial models to changes in gas concentrations over time. In order to avoid bias associated with the initial stabilization period and saturation towards the end of the measurements, only data measured from 50-250 sec were included in these calculations, and gas fluxes were calculated from slopes taken 100 sec after the start of each measurement.
The copy number of bacterial 16S ribosomal RNA genes in DNA extracts from frost boil soil was measured in 20 µl reactions, consisting of 0.8 µl of each of the primers 341F (5'-CCTAYGGGRBGCASCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'),10 µl of 2 x qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems Inc., Wayne, PA, USA), 2 µl of sample (diluted 10 times to avoid inhibition of PCR) and 6.4 µl of H2O. The PCR mixes were heated to 95 °C for 180 sec, and then subjected to 45 cycles of 95 °C for 5 sec and a final melt at 60 °C for 30 sec on LightCycler® 96 real-time PCR instrument. Fungal ITS2 copy numbers were measured in the same way, but with 0.8 µl of each of the primers ITS4 (5'-TTCCTSCGCTTATTGATATGC-3') and ITS7 (5'-GTGARTCATCGARTCTTTG-3') in 20 µl reactions. The measurements from one sample, for which the copy numbers of bacterial 16S ribosomal RNA genes and fungal ITS2 regions were 2-3 orders of magnitude lower than the other 47 samples, were deleted from the dataset. Copy numbers were expressed per g dry weight of soil (105 °C for 18 h).
DNA extracted from soil was suspended in 50 µl of Tris-EDTA buffer. A universal eubacterial primer set, 331F (5'-TCCTACGGGAGGCAGCAGT-3')/ 797R (5'-GGACTACCAGGGTATCTAATCCTGTT-3'), was used to amplify V3-V4 hypervariable regions of the 16S ribosomal RNA gene with MiSeq adapters. Each PCR was carried out in a 50 µl reaction volume containing 2 x Premix Taq, 1 µM of each primer and 1 µl of DNA extract. PCR amplification was performed in a BioRad T100 thermal cycler with an initial denaturation step at 95 °C for 5 min, followed by 35 cycles of denaturation for 15 sec at 95 °C, annealing for 45 sec at 56 °C and elongation for 90 sec at 72 °C, and a final elonga...(20)
Data collection:
Equipment
Soil temperatures: Tinytag Plus 2 loggers (TGP-4017, Gemini Data Loggers Ltd., Chichester, UK)
Greenhouse gas exchange: Piccaro Gas Analyzer (Picarro G4301, Santa Clara, CA, USA)
Q-PCR assays: LightCycler 96 real-time PCR instrument (Roche Life Science, Hvidovre, Denmark)
PCR amplification: BioRad T100 thermal cycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA)
DNA barcoding: Illumina MiSeq sequencer (Illumina, Inc., San Diego, CA, USA)
Software
Trimmomatic version v. 0.35
mothur v1.40.5
Data quality:
Temperature data are the means derived from loggers in four unchambered plots and in four chambered plots.
Gas exchange data are means of two measurements taken from each plot on 2018-08-23 and 2018-08-26.
Missing data are indicated by NA.
Metadata
- File identifier
- 71dfd312-a493-42fc-822a-29f3dca66dc0 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Hierarchy level name
-
dataset
- Date stamp
- 2022-09-14
- Metadata standard name
-
ISO 19115 Geographic Information - Metadata
- Metadata standard version
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ISO 19115:2003(E)
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Point of contact
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