7abe787a-4561-4715-8b49-983faa4c0787 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:24:19 UK GEMINI 2.3 Levels of the pheromone 7,11-heptacosadiene in the sibling species Drosophila simulans and Drosophila sechellia 2023-02-20 publication 2023-02-15 creation https://catalogue.ceh.ac.uk/id/7abe787a-4561-4715-8b49-983faa4c0787 10.5285/7abe787a-4561-4715-8b49-983faa4c0787 doi: Erezyilmaz, D., Doubovetzky, N., Billeter, J.C., Goodwin, S.F. (2023). Levels of the pheromone 7,11-heptacosadiene in the sibling species Drosophila simulans and Drosophila sechellia. NERC EDS Environmental Information Data Centre 10.5285/7abe787a-4561-4715-8b49-983faa4c0787 This dataset provides measurements of the cuticular pheromone, 7,11-heptacosadiene of individual female vinegar flies. The purpose of the dataset is to compare the levels between two sibling species, Drosophila simulans and Drosophila sechellia. Single female virgins were isolated from males at eclosion, aged five days, and then washed in hexane to extract cuticular hydrocarbons. The extracts were analyzed by gas chromatography relative to an internal standard. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/7abe787a-4561-4715-8b49-983faa4c0787 Erezyilmaz, D. University of Oxford deniz.erezyilmaz@dpag.ox.ac.uk https://orcid.org/0000-0001-8416-8561 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Doubovetzky, N. University of Groningen nicolasdoubo@hotmail.com https://orcid.org/0000-0001-6219-3125 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Billeter, J.C. University of Groningen j.c.billeter@rug.nl https://orcid.org/0000-0001-7796-4068 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Goodwin, S.F. University of Oxford Stephen.goodwin@dpag.ox.ac.uk https://orcid.org/0000-0002-0552-4140 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Erezyilmaz, D. University of Oxford deniz.erezyilmaz@dpag.ox.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Oxford EMAIL NOT PROVIDED owner Cuticular hydrocarbons contact pheromone Wikidata 2012-10-29 creation long-chain fatty acids Drosophila courtship gas chromatography otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Erezyilmaz, D., Doubovetzky, N., Billeter, J.C., Goodwin, S.F. (2023). Levels of the pheromone 7,11-heptacosadiene in the sibling species Drosophila simulans and Drosophila sechellia. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/7abe787a-4561-4715-8b49-983faa4c0787 English utf8 biota environment Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/7abe787a-4561-4715-8b49-983faa4c0787 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/7abe787a-4561-4715-8b49-983faa4c0787.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Drosophila females that were five days old were anaesthetised on ice for ten minutes. Individual female flies were then transferred to 150uL pure HPLC grade hexane within a 200uL glass vial that was fitted with a glass insert. The vial with the fly inside was vortexed to extract the hydrocarbons. Afterwards the fly was removed, the hexane extract was dried down under a gentle flow of nitrogen, and the vial was shipped to Groningen for gas chromatograph analysis. The retention time in minutes of each peak relative to an internal standard (C26), was used to measure size and identify 7,11-heptacosadiene. The area underneath each peak, relative to the standard was used to measure the quantity of 7,11-heptacosadiene. ChemStation software (Agilent technologies) was used to estimate area under the peak.