7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2023-06-18 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Measurements for a 92 day climate manipulation experiment on replicate macrofaunal mesocosms collected on the cruise JR18006 in the Western Barents Sea and at Rothera research station in 2019 2023-06-18 creation 2023-06-18 revision 2023-06-18 publication 2023-06-18 released 1.0 https://doi.org/10.5285/7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 doi GB/NERC/BAS/PDC/01749 https://data.bas.ac.uk/ NE/S007210/1 award NE/N015894/1 award NE/P006426/1 award Please cite this item as: Williams, T.J., Reed, A.J., Peck, L.S., Godbold, J.A., & Solan, M. (2023). Measurements for a 92 day climate manipulation experiment on replicate macrofaunal mesocosms collected on the cruise JR18006 in the Western Barents Sea and at Rothera research station in 2019 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 Measurements were collected during the 92 day lab-based experiment carried out on replicate macrofaunal mesocosms of Astarte crenata, Ctenodiscus crispatus, Cistenides hyperborea from the Western Barents Sea and Aequiyoldia eightsi and Laternula elliptica from Rothera Point, Antarctic Peninsula. Macrofauna were collected on cruise JR18006 in the Western Barents Sea and by hand during scuba dives in the cove adjacent to Rothera research station. Experimental measurements include carbonate chemistry indices measured in water samples; carbon dioxide concentration levels; benthic invertebrate response rate, burial rate, sediment particle reworking, bioirrigation, and associated nutrient concentrations; benthic invertebrate body size and individual biomass; sediment organic material content, and sediment particle size. Supported by the INSPIRE Doctoral Training Partnership programme (grant NE/S007210/1) and 'The Changing Arctic Ocean Seafloor (ChAOS) - how changing sea ice conditions impact biological communities, biogeochemical processes and ecosystems' project (NE/N015894/1 and NE/P006426/1, 2017/2021), Natural Environment Research Council (NERC) in the UK. No credit. completed Williams, Thomas J. University of Southampton Researcher author Reed, Adam J. University of Southampton Researcher author Peck, Lloyd S. British Antarctic Survey Researcher author Godbold, Jasmin A. University of Southampton Researcher author Solan, Martin University of Southampton Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Ocean Chemistry > Carbon Dioxide EARTH SCIENCE > Oceans > Ocean Temperature theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctic Arctic bioirrigation bioturbation climate change ecosystem function experiment mesocosm experiment theme Hamble, UK Barents Sea, Antarctic Barents Sea Rothera Point, Antarctic Peninsula Antarctica place Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions No restrictions apply. url url dependency doi doi crossReference url url crossReference textTable eng utf8 biota 1.3 1.3 50.9 50.9 2019-09-21 2020-01-21 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv application/pdf bytes 726663168 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 WWW:LINK-1.0-http--link Get Data Download data download bytes 726663168 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 WWW:LINK-1.0-http--link Get Data Download data download bytes 726663168 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Individuals of Astarte crenata, Ctenodiscus crispatus and Cistenides hyperborea were obtained from replicate SMBA (Scottish Marine Biological Association) box cores and 15 minute Agassiz trawls in the Barents Sea (stations B13, 74.3 degrees N, 30.0 degrees E; B16, 80.3 degrees N, 30.0 degrees E; JCR18006, RSS James Clark Ross) in July 2019. Individuals of the Aequiyoldia eightsi and Laternula elliptica were collected by SCUBA-assisted divers at Rothera Point, Adelaide Island, West Antarctic Peninsula (67.3 degrees S, 68.1 degrees W) in March-April 2019. Surficial sediment (less than 5 cm depth) for the Arctic species was collected using SMBA box cores at stations B13, B14 (76.3 degrees N, 30.3 degrees E) and B16 in July 2019 and, for the Antarctic species, by hand from the Hamble, UK (50.9 degrees N, 1.3 degrees W). Sediment was sieved (500 micrometer mesh) within a seawater bath to retain the fine fraction and remove macrofauna and debris. Species were distributed across 42 clear acrylic aquaria (internal LWH: 12 x 12 x 33 cm, 3 replicates treatment-1: species × location × climate scenario), designed to accommodate the appropriate density (Arctic, 2 ind. mesocosm-1; Antarctic, 1 ind. Mesocosm-1; size and burrowing requirements of each species (sediment depth: A. crenata, C. crispatus & C. hyperborea, 16 cm; A. eightsi, 12cm; L. elliptica, 19cm), and exposed to ambient (1 ± 0.5°C, around 400 ppm [CO2]) and indicative near-future (3 +/- 0.5 degrees Celsius, around 550 ppm [CO2]) environmental conditions. Aquaria were randomly placed within one of two insulated seawater reservoirs (LWH: 1.2 x 1.2 x 0.8m, Tanks Direct, UK). All aquaria were overlain with seawater (salinity 33, 10 micrometer sand filtered, UV sterilized) to a combined sediment and water depth of 31 cm and maintained in the dark. After acclimation to ambient mesocosm conditions (21 days, 09-29/09/2019), the water temperature and atmospheric CO2 of the aquaria in the future treatment was gradually increased at 0.5 degrees Celsius and 50 ppm increments every 7 days to reach the desired near-future climate conditions. During both the acclimation and experimental period (92 days, 21/10/2019 - 21/01/2020), C. crispatus and C. hyperborea were fed ad libitum once a week with commercially available fish food (Aquarian Tropical Flake; 0.03 g), and A. crenata, A. eightsi and L. elliptica were fed ad libitum three times per week with 100 ml of precultured phytoplankton (33:33:33 mix, Isochrysis sp., Tetraselmis sp., and Phaeodactylum sp.; Algal Culture Laboratory, National Oceanography Centre Southampton). To avoid accumulation of excess food and associated nutrients, partial seawater changes (weekly 50 percentage seawater exchange) were undertaken. Temperature, pH, salinity and total alkalinity were periodically measured across all experimental mesocosms (T,pH,S: weekly, AT: week 2, week 6, week 11). Total alkalinity analysis followed the standard HCl titration protocol of the Carbonate Facility at the University of Southampton. From which, DIC, [pCO2], [calcite], [aragonite], [HCO3] and [CO3] were calculated using the CO2calc software. CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption were measured continuously in the ambient air and future climate regime gas mixture from the start (21/10/2019) to end (21/01/2020) of the experimental period. All measurements were calculated using an Infra-red gas analyzer LiCOR LI-820 model and recorded using the interface software on a standard laptop. Behavioural activity was quantified using measurements of movement and burial behaviour at the sediment surface. Individuals (morphology, +/- 0.01 mm; blotted wet weight, +/- 0.001 g) were placed in temperature-acclimatised viewing trays containing sediment (depth 5 cm) overlain with sea water (depth 3 cm) and viewed (less ...(31) Data collection: Behaviour: Logitech C920 HD Pro Webcam, 1080p Behaviour: SkyStudioPro time-lapse software V 1.1.0.30 Behaviour: VLC Media Player V 3.0.12 Sediment particle reworking: Canon 400D digital SLR camera Sediment particle reworking: ImageJ (version 1.47s) Bioirrigation: Tecator flow injection auto-analyser (FIA Star 5010 series) Nutrients: Lachat Quikchem 8500 flow-injection auto-analyser Total Alkalinity: Apollo SciTech Alkalinity Titrator AS-ALK2 Salinity: WTW (TM) TetraCom (TM) 325 Standard temperature-conductivity combination electrode Temperature: NBS scale, Mettler-Toledo InLab Expert Pro temperature-pH combination electrode pH: NBS scale, Mettler-Toledo InLab Expert Pro temperature-pH combination electrode pCO2, CO3, HCO3, Ωcalcite, Ωaragonite, TCO2: Calculated using the CO2calc software [version 4.0.9] CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption : Infra-red gas analyzer LiCOR Li-820 CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption: ACER laptop CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption: LiCOR Windows software Sediment: Malvern Mastersizer 2000 He-Ne LASER diffraction sizer Data quality: Standard protocols were followed and data entry double checked by independent person. Missing values for behavioural activity (response rate, burial rate) relate to the individual not completing the associated activity within the 60 minute timeframe. Missing biomass data is due to mortalities. Original unedited raw videos and processed, stitched together timelapses are provided. Original unedited raw fSPI images and processed, stitched together fSPI profiles are provided. 3 replicate water samples were taken for each independent treatment level in the experiments. Standards and blanks were utilised when analysing total alkalinity as per the HCl protocol. Standard protocols were followed and data entry double checked by independent person. LiCOR instruments were calibrated 3 months prior to the start of the experimental period. asNeeded completed