BGS Hosted Metadata
DNA metabarcoding, plant community and soil environmental data from a 15-month DNA application experiment in southern Iceland, June 2023-October 2024
The datasets originate from a field experiment designed to assess the mobility and longevity of DNA in a soil environment. We applied radish DNA - in the form of leaves (solid treatment) and PCR product (liquid treatment) to experimental plots in southern Iceland. We sampled the soil from different depths under the plots over a period of 15 months, looking for traces of radish DNA. The dataset encompasses 1) DNA metabarcoding data (plants) derived from soil samples; 2) a survey of extant plant communities; 3) a record of near-surface soil temperatures for the duration of the experiment; and 4) data quantifying the decomposition of radish leaves applied to the plots. The radish DNA was applied to six experimental plots in June 2023; soil samples were collected from below the plots in May 2024 (M24) and October 2024 (O24). DNA extraction and PCR was conducted by Dr Doreen Huang (University of Southampton). DNA from the soil samples was sequenced by a commercial third party (Novogene). Full details about this dataset can be found at https://doi.org/10.5285/7c6cbb74-96ad-4269-bad7-51025963ef23
Simple
- Date (Publication)
- 2025-09-12
- Citation identifier
- https://catalogue.ceh.ac.uk/id/7c6cbb74-96ad-4269-bad7-51025963ef23
- Citation identifier
- doi: / 10.5285/7c6cbb74-96ad-4269-bad7-51025963ef23
- Other citation details
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Cutler, N., Huang, D.Y., Dugmore, A., Streeter, R. (2025). DNA metabarcoding, plant community and soil environmental data from a 15-month DNA application experiment in southern Iceland, June 2023-October 2024. NERC EDS Environmental Information Data Centre 10.5285/7c6cbb74-96ad-4269-bad7-51025963ef23
- Point of contact
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Organisation name Individual name Electronic mail address Role Newcastle University Nick Cutler
Point of contact Newcastle University Cutler, N. Author University of Southampton Huang, D.Y. Author University of Edinburgh Dugmore, A. Author University of St Andrews Streeter, R. Author Newcastle University EMAIL NOT PROVIDED
Owner NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher
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GEMET - INSPIRE themes, version 1.0
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Soil
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- Keywords
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- Environmental survey
- Biodiversity
- Soil
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Iceland
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Environmental DNA
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Palaeoenvironmental reconstruction
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Decomposition experiment
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Soil moisture
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Cutler, N., Huang, D.Y., Dugmore, A., Streeter, R. (2025). DNA metabarcoding, plant community and soil environmental data from a 15-month DNA application experiment in southern Iceland, June 2023-October 2024. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/7c6cbb74-96ad-4269-bad7-51025963ef23
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Begin date
- 2023-06-01
- End date
- 2024-10-31
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/7c6cbb74-96ad-4269-bad7-51025963ef23.zip Supporting information
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/7c6cbb74-96ad-4269-bad7-51025963ef23 Download the data
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Field data: The radish DNA was applied to six experimental plots in June 2023 as radish leaves and liquid PCR product. Replicate soil samples were collected from depths of 5 cm and 20 cm below the plots in October 2023 and May 2024. Samples were collected from the soil surface in September 2024, after which the experiment was discontinued. Soil temperatures were recorded for 332 days at hourly intervals using an iButton soil temperature logger. The decomposition of the radish leaves was calculated by weighing the mesh bags carrying the leaves at the beginning and end of the experiment. Plant community composition was evaluated by a visual estimate of proportional cover (Domin scale) within each plot. Laboratory analysis: DNA was extracted using DNeasy PowerSoil Pro Kit (QIAGEN). Plant DNA was amplified in six replicate PCRs. After PCR, the samples and PCR negatives were pooled and purified with QIAquick PCR purification kit (QIAGEN). PCR-free library preparation was carried out by a commercial DNA sequencing company (Novogene) on an Illumina NovaSeq 6000 PE250 platform.
Metadata
- File identifier
- 7c6cbb74-96ad-4269-bad7-51025963ef23 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:22:29
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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