Date (Creation)

2021-02-08

Date (Revision)

2021-02-08

Date (Publication)

2021-02-08

Date (released)

2021-02-08

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/80de87f3-9497-4606-8c7d-3293ab05651f

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01463

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Newsham, K., Goodall-Copestake, W., & Foot, G. (2021). Microscopy data on fungal structures in the tissues of leafy and simple thalloid II liverwort species sampled from High Arctic Spitsbergen (Version 1.0) [Data set]. UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation. https://doi.org/10.5285/80de87f3-9497-4606-8c7d-3293ab05651f

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey, Natural Environmental Research Council, UK Research & Innovation	Newsham, Kevin		Author
British Antarctic Survey, Natural Environmental Research Council, UK Research & Innovation	Goodall-Copestake, William		Author
British Antarctic Survey, Natural Environmental Research Council, UK Research & Innovation	Foot, George		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Fungi

Theme

• Jungermanniidae

• Metzgeriidae

• Svalbard

• leafy liverwort

• mycothalli

• simple thalloid II liverwort

• symbiosis

Place

• South Georgia

• Spitsbergen

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data released under Open Government Licence V3.0:

Use constraints

Other restrictions

Other constraints

No restrictions apply.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2010-01-07

End date

2015-08-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

In July 2010, August 2011, July 2012 and August 2015, 140 specimens of 13 leafy liverwort species were collected from eight sites on Broggerhalvoya and Blomstrandhalvoya on Spitsbergen in the High Arctic archipelago of Svalbard.The plants were dried at room temperature and were subsequently rehydrated in water and cleared in 10% KOH for 24 h. They were then rinsed thoroughly with water and were bleached in a mixture of 30% hydrogen peroxide and 3% ammonium hydroxide (10:1 v/v) for up to 10 min. The plants were then washed thoroughly with water, were acidified in 5% lactic acid for 1 h and were stained with 0.01% aniline blue in lactic acid for 24 h. They were then removed from the staining solution, excess stain was drawn off on absorbent paper, and the plants were destained for at least 24 h in 80% lactic acid. Between 10 and 15 stems of each species, and at least 40 stems of C. varians and C. bicuspidata, were then lightly squashed in 80% lactic acid on glass slides prior to observation under UV epifluorescence at x 400 magnification (BX51 microscope, Olympus UK Ltd). Using the line intersection method described by McGonigle et al. (1990, New Phytologist 155, 495-501), the percentages of stem length colonised by hyphal coils (in rhizoid bases and stem epidermal cells), hyaline septate hyphae (in stem epidermal cells) and dark septate hyphae (on stem surfaces) were calculated. At least 50 intersections were scored in each specimen. The percentage of c. 30 rhizoids in each specimen that were colonised by hyaline hyphae was also recorded.

Data collection:

BX51 microscope, Olympus UK Ltd

Data quality:

Raw data are shown. The data have not been cleaned. Missing data in the 'Rhizoids_colonised_percentage' column (indicating that no rhizoids were observed on the stems of plants) are indicated by NA.

Metadata

File identifier

80de87f3-9497-4606-8c7d-3293ab05651f XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2021-02-08

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact