82721bfc-d707-4255-bc5b-4d5882f9881b English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-12-02T08:27:57 UK GEMINI 2.3 Physiological measurements and root morphology of poplar trees cultivated with different fungal guilds 2025-09-18 publication https://catalogue.ceh.ac.uk/id/82721bfc-d707-4255-bc5b-4d5882f9881b 10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b doi: Pena, R., Tibbett, M. (2025). Physiological measurements and root morphology of poplar trees cultivated with different fungal guilds. NERC EDS Environmental Information Data Centre 10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b This dataset encompasses 1) Detailed measurements of root architecture parameters - numerical values for Total length (cm), Area (cm²), Surface area (cm²), Average diameter (mm); Root volume (cm³), Number of tips, forks, and crossings; and 2) The physiological responses of poplar trees under varying light conditions - measurements of Transpiration rate (mmol m⁻² s⁻¹), Net CO₂ assimilation rate (µmol m⁻² s⁻¹), Intercellular CO₂ concentration (µmol mol⁻¹), Intercellular CO₂ partial pressure (Pa), and Stomatal conductance to water vapour (mol m⁻² s⁻¹). Data were generated in a glasshouse experiment under elevated CO₂ (740 µmol mol⁻¹). The Root Architecture data was collected when the trees were six months old. Net Photosynthesis Light Curves were recorded at four time points, starting when the poplar trees were two months old and continuing until they were six months old. The root architecture data were obtained through WinRhizo scanning and software (Regent Instruments Inc., Quebec City, QC, Canada). The physiological measurements were recorded using LI-6800P Portable Photosynthesis System (LI-COR Biosciences, Lincoln, NE, USA). To construct the light curves, measurements were taken starting from a maximum light intensity of 1500 PPFD (µmol m⁻² s⁻¹) and subsequently decreased to 800, 200 and 50 µmol m⁻² s⁻¹ until no light was applied. In this experiment, micro-propagated poplar clones were cultivated under four different conditions: without fungi (fungal-free plants), with ectomycorrhizal fungi (ectomycorrhizal plants), with saprotrophic fungi (saprotrophic fungal plants), and with both ectomycorrhizal and saprotrophic fungi (fungal plants). The purpose of the experiment was to evaluate plant performance under the influence of different fungal guilds and their interactions. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b Rodica Pena University of Reading rodica.pena@reading.ac.uk pointOfContact Pena, R. University of Reading rodica.pena@reading.ac.uk https://orcid.org/0000-0002-7985-6906 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Tibbett, M. University of Reading m.tibbett@reading.ac.uk https://orcid.org/0000-0003-0143-2190 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Reading EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Soil Biodiversity WinRhizo light curves ectomycorrhiza saprotrophs poplar clone otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Pena, R., Tibbett, M. (2025). Physiological measurements and root morphology of poplar trees cultivated with different fungal guilds. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/82721bfc-d707-4255-bc5b-4d5882f9881b English utf8 biota 2023-06-01 2023-12-01 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/82721bfc-d707-4255-bc5b-4d5882f9881b Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/82721bfc-d707-4255-bc5b-4d5882f9881b.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The plant material used was Populus canescens (grey poplar), a hybrid between Populus tremula and Populus alba. The poplar clones were initially aseptically cultivated in Murashige and Skoog (MS) medium and subsequently transferred to a sandy substrate. The trees were harvested at six months old. The entire root system was collected and immediately scanned using the WinRhizo system. The obtained images were automatically processed using the device's internal software, ensuring accuracy and consistency. No additional data processing was required. Physiological measurements were conducted regularly at four time points during the growth period in the sandy substrate, from when the trees were two months old until they reached six months. Data were collected and processed using the LI-6800P system's internal software, ensuring no additional data processing was necessary. Once collected, the data were controlled to ensure integrity and accuracy and then transferred to the data bank.