BGS Hosted Metadata
Genotype-phenotype association for mate choice in a Drosophila simulans-sechellia backcross population
The data resource contains Drosophila simulans-sechellia backcross female mate choice (phenotype) aligned with whole-genome genotypes of 692 female backcross progeny. The data was generated in a laboratory at Stony Brook University New York and each test was conducted in 28.5 x 95mm plastic vials. Phenotype data was collected in 2015 and the final analysis was completed in 2021. Illumina libraries were sequenced at University of Oregon G3 Genomics Center, and the multiplexed shotgun genotyping (MSG) software was run at Janelia Research Campus Virginia. The experiments were conducted to align the genotype of females with behavioural phenotype, female preference. Three test females were allowed to choose from three males of either species. ‘Choice’ was determined by copulation, and the species of ‘chosen’ male was recorded before gDNA was extracted from the female. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing. Deniz Erezyilmaz was responsible for data collection and interpretation. Those genomes with ambiguous genotypes at more than 25% of markers due to sparse reads/coverage were eliminated from the analysis. The research was funded through NERC grant NE/S010351/1. Investigating the dual role of mate choice genes in behavioural isolation and hybridization. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb
Simple
- Date (Publication)
- 2022-04-13
- Citation identifier
- https://catalogue.ceh.ac.uk/id/871c537d-2a84-432f-8597-7e895e0ffdcb
- Citation identifier
- doi: / 10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb
- Other citation details
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Erezyilmaz, D.F., Lu, K. (2022). Genotype-phenotype association for mate choice in a Drosophila simulans-sechellia backcross population. NERC EDS Environmental Information Data Centre 10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Oxford Erezyilmaz, D. Point of contact University of Oxford Erezyilmaz, D.F. Author State University of New York Lu, K.
Author University of Oxford Erezyilmaz, D.F.
Owner NERC EDS Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian
- Maintenance and update frequency
- Not planned
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Wikidata
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Erezyilmaz, D.F., Lu, K. (2022). Genotype-phenotype association for mate choice in a Drosophila simulans-sechellia backcross population. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/871c537d-2a84-432f-8597-7e895e0ffdcb
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/871c537d-2a84-432f-8597-7e895e0ffdcb Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/871c537d-2a84-432f-8597-7e895e0ffdcb.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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The Drosophila simulans-sechellia backcross genotypes were obtained by Multiplexed Shotgun Genotyping (Andolfatto et al, 2011), and the files were thinned using David Stern’s (Janelia Research Campus) script, pull_thin (github.com/dstern/pull_thin) were obtained by two-choice assay, females choosing between D. simulans and D. sechellia males. Standard molecular biology was used to generate Illumina libraries from individual backcross females for sequencing, as described in: Andolfatto, P., Davison, D., Erezyilmaz, D., Hu, T.T., Mast, J., Sunayama-Morita, T., & Stern, D.L. (2011). Multiplexed shotgun genotyping for rapid and efficient genetic mapping. In Genome Research (Vol. 21, Issue 4, pp. 610–617). Cold Spring Harbor Laboratory. https://doi.org/10.1101/gr.115402.110
Metadata
- File identifier
- 871c537d-2a84-432f-8597-7e895e0ffdcb XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-11-13T16:20:54
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
