87fd0d61-7405-4a66-9620-2be10c25d452 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2022-08-05 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Microscopy data on mycothalli in 16 leafy liverwort species from the Thatcher Peninsula, South Georgia 2022-08-05 creation 2022-08-05 revision 2022-08-05 publication 2022-08-05 released 1.0 https://doi.org/10.5285/87fd0d61-7405-4a66-9620-2be10c25d452 doi GB/NERC/BAS/PDC/01655 https://data.bas.ac.uk/ Please cite this item as: Newsham, K. (2022). Microscopy data on mycothalli in 16 leafy liverwort species from the Thatcher Peninsula, South Georgia (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/87fd0d61-7405-4a66-9620-2be10c25d452 Microscopy data on the percentages of liverwort stem length colonised by (i) stained hyphal coils, (ii) stained septate hyphae and (iii) dark septate hyphae, and (iv) percentages of rhizoids colonised by hyphae, in 16 leafy liverwort species sampled from sub-Antarctic South Georgia. Specimens were collected in 2011 and 2016 from 12 sites on the Thatcher Peninsula, South Georgia. The specimens have been deposited in the British Antarctic Survey herbarium. This project was funded by NERC under the British Antarctic Survey Long Term Monitoring programme. No credit. completed Newsham, Kevin K British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Ecological Dynamics > Symbiosis EARTH SCIENCE > Biosphere > Fungi theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Jungermanniidae South Georgia leafy liverwort mycothalli symbiosis theme Thatcher Peninsula South Georgia Island place Habitats and biotopes theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/ otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions No restrictions apply url url crossReference textTable eng utf8 biota -36.49675 -36.59533 -54.2978 -54.26108 2011-11-01 2016-02-29 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/csv bytes 7168 https://ramadda.data.bas.ac.uk/repository/entry/show?entryid=87fd0d61-7405-4a66-9620-2be10c25d452 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: In October to November 2011 and January to February 2016, 83 specimens of 16 leafy liverwort species in the Adelanthaceae, Anastrophyllaceae, Cephaloziaceae, Cephaloziellaceae, Lophoziaceae, Lophocoleaceae, Plagiochilaceae and Schistochilaceae were collected from 12 sites on the Thatcher Peninsula. The specimens, which were air dried at room temperature in clean paper packets within several hours of collection, have been deposited in the British Antarctic Survey herbarium (AAS see https://data.bas. ac.uk/metadata.php?id=GB/NERC/BAS/AEDC/00023). Dried plants were rehydrated in water and cleared in 10% KOH for 24 h at room temperature. All plants were then rinsed thoroughly with water and were bleached in a mixture of 30% hydrogen peroxide and 3% ammonium hydroxide (10:1 v/v) for up to 10 min. The plants were then washed thoroughly with water, were acidified in 5% lactic acid for 1 h and were stained with 0.01% aniline blue in lactic acid for 24 h. They were then removed from the staining solution, excess stain was drawn off on absorbent paper, and the plants were destained for at least 24 h in 80% lactic acid. From each sample, between 10 to 20 stems of each species, and at least 40 stems of Cephalozia badia, Cephaloziella varians and Clasmatocolea humilis, were then lightly squashed in 80% lactic acid on glass slides prior to observation under UV epifluorescence at x400 magnification (BX51 microscope, Olympus UK Ltd). The line intersection method described by McGonigle et al. (1990) was used to calculate the percentages of stem length colonised (SLC) by stained hyphal coils in rhizoid bases and stem epidermal cells, stained septate hyphae in stem epidermal cells, dark septate hyphae on stem surfaces, and dark septate coils in rhizoid bases and stem epidermal cells. A total of 4,442 intersections were examined, with up to 119 intersections being scored in each sample. The percentage of at least 30 rhizoids in each specimen that were colonised by hyphae was also recorded, with a total of 2,541 rhizoids being examined. Insufficient rhizoids were observed on the stems of Chiloscyphus koeppensis or Pachyglossa spegazziniana to derive means for rhizoid colonisation. Instrumentation: BX51 compound microscope fitted with a UPlanApo x40 objective lens, a 100-W mercury short arc lamp and an ultraviolet fluorescence filter cube (U-MWU2, consisting of a BP 330-385 excitation filter, a DM 400 dichromatic mirror and an LP 420 emission filter; Olympus Life Science, Tokyo, Japan). Data collection: BX51 compound microscope fitted with a UPlanApo x40 objective lens, a 100-W mercury short arc lamp and an ultraviolet fluorescence filter cube (U-MWU2, consisting of a BP 330-385 excitation filter, a DM 400 dichromatic mirror and an LP 420 emission filter; Olympus Life Science, Tokyo, Japan). Data quality: Raw data are shown. The data have not been cleaned. Missing data in the Rhizoids colonised (%) column (indicating that no rhizoids were observed on the stems of plants) are indicated by NA. asNeeded completed