BGS Hosted Metadata
Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014
These data consist of relative telomere length (RTL) measures from quantitative polymerase chain reaction, of Seychelles warbler birds on Cousin Island, Seychelles. The data were collected by the Seychelles Warbler Project in 1995-2014. Data include bird identity, sex, age, birth period, qPCR plate identity, RTL, technician, territory, field period, mum ID, dad ID, mum age at conception, dad age at conception, dominant female ID in the natal territory, dominant male ID in the natal territory Full details about this dataset can be found at https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
Simple
- Date (Publication)
- 2021-07-26
- Date (Creation)
- 2021-06-24
- Citation identifier
- https://catalogue.ceh.ac.uk/id/8a8240a2-e8ed-495d-ae93-c35200956764
- Citation identifier
- doi: / 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
- Other citation details
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Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre 10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Leeds
Sparks, A.M. Author University of East Anglia
Spurgin, L.G. Author University of Groningen
van der Velde, M.
Author University of East Anglia
Fairfield, E.A.
Author University of Groningen
Komdeur, J.
Author University of Sheffield
Burke, T. Author University of East Anglia
Richardson, D.S. Author University of Groningen
Dugdale, H.L. Author University of Groningen
Dugdale, H. Point of contact NERC EDS Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian University of Leeds
Dugdale, H. Owner
- Access constraints
- Other restrictions
- Other constraints
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Controlled
- Use constraints
- Other restrictions
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If you reuse this data, you should cite: Sparks, A.M., Spurgin, L.G., van der Velde, M., Fairfield, E.A., Komdeur, J., Burke, T., Richardson, D.S., Dugdale, H.L. (2021). Telomere length data from a wild population of Seychelles Warblers on Cousin Island, 1995-2014. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/8a8240a2-e8ed-495d-ae93-c35200956764
- Spatial representation type
- Text, table
- Distance
- 1 urn:ogc:def:uom:EPSG::9001
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Begin date
- 1995-01-01
- End date
- 2014-12-31
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/8a8240a2-e8ed-495d-ae93-c35200956764 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/8a8240a2-e8ed-495d-ae93-c35200956764.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Blood samples (~25 μl) were collected and stored at room temperature in absolute ethanol. DNA was extracted using a DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s instructions with modification of overnight lysis at 37 °C and a final DNA elution volume of 80 μl. DNA concentration and purity were quantified using a NanoDrop 8000 Spectrophotometer (ThermoScientific). The following thresholds were applied before samples were included for further analysis: (1) DNA concentration must be at least 15 ng/μl (based on a mean of three measurements), (2) the 260/280 absorbance ratio has to be between 1.8 and 2.0 for acceptable DNA purity, and (3) the 260/230 absorbance ratio must be higher than 1.8. DNA integrity was further validated by visualisation with ethidium bromide after electrophoresis on a 1.2% agarose gel, and all samples with evidence of DNA degradation were re-extracted or excluded. All DNA extractions that passed the above criteria were diluted to 3.3 ng/μl before telomere measurement. We used quantitative polymerase chain reaction to calculate a relative measure of telomere length that describes the amount of telomeric DNA in a sample relative to that of GAPDH, a constantly expressed reference gene.
Metadata
- File identifier
- 8a8240a2-e8ed-495d-ae93-c35200956764 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
-
dataset
- Date stamp
- 2025-04-08T10:43:40
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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