Date (Creation)

2025-05-13

Date (Revision)

2025-05-13

Date (Publication)

2025-05-13

Date (released)

2025-05-13

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/9c459656-5fe4-44f7-860f-da287111016c

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/02060

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Romero Martinez, M.L., Hollyman, P., & Collins, M. (2025). Fish bycatch diversity within the Antarctic krill fishery, CCAMLR sub-areas 48.1 to 48.3, 2019-2024 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/9c459656-5fe4-44f7-860f-da287111016c

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Romero Martinez, Maria Lorena		Author
Bangor University	Hollyman, Philip		Author
British Antarctic Survey	Collins, Martin		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Agriculture > Agricultural Aquatic Sciences > Fisheries

Theme

• Antarctic Peninsula

• Area 48

• Fish

• Krill bycatch

• South Georgia

Place

• South Georgia Island

• South Orkney Islands

• South Shetland Islands

• Antarctic Peninsula Antarctica

GEMET - INSPIRE themes, version 1.0

• Agricultural and aquaculture facilities

Access constraints

Other restrictions

Other constraints

public access limited according to Article 13(1)(e) of the INSPIRE Directive

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

and supplied under Open Government Licence v.3 ().

Use constraints

Other restrictions

Other constraints

Data is under embargo until the publication of an associated paper.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Larger work citation

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2022-11-01

End date

2024-10-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Samples were collected by fishery observers as part of the 25kg per trawl collected for the reporting of fish bycatch with the Antarctic krill fishery. The spatial distribution for the collection of samples covered the three subareas of Area 48 delimited by CCAMLR, subarea 48.1 (Antarctic Peninsula), 48.2 (Southern Scotia Sea) and 48.3 (Northern Scotia Sea including South Georgia Island).

To process the samples an integrative taxonomy approached taken, whereby each specimen was identified to the lowest taxonomic level following the morphological keys provided in Efremenko (1983) and Kellermann (1989) for larval fish, while for juvenile and adult fish the morphological keys in Gon and Heemstra (1990) and Collins and Xavier (2022) were used.

Total and standard length were measured and a photographed from the left side of the fish was taken. Photographic material was produced by mounting a Nikon Z camera fitted with a macro lens on a Kaiser copy stand.

Each specimen was subsampled for DNA by making a small incision on the right side of the fish above the lateral line to take a small piece of tissue (25mg) as well as a fin clip (whenever possible). Tissue samples were then stored in 90% ETOH at -20°C before the DNA extraction and PCR amplification of cox1 gen and control region with species-specific primers.

DNA was extracted from 25mg of muscle tissue using two types of kit and following manufactures instructions. The extracted gDNA was resuspended in 50 to 70 micro l eluent and stored at -20 degrees °C before amplification of targeted genes by polymerase chain reaction (PCR). Species specific primers were designed for the PCR amplification of cox1 and control region (Romero Martínez et al., 2023 and 2024).

PCR amplification was performer on a thermocycle, PCR reactions mix consisted of 3-5 ng of DNA template, 7.5 micro l of master mix, 0.3-0.5 micro M of each forward and reverse primer to make a 15 micro l reaction. The thermocycling profile was 2 min at 95°C inital denaturation followed by 38 cycles at 95°C for 20 s, 50°C for 30 s, 72°C for 1:30 min and a final extension at 72°C for 1 min for cox1 and inital denaturation at 95°C for 5 min, 38 cycles at 95°C for 20 s, 50-55°C for 30 s, 72°C for 2 min, and final extension at 72°C for 1 min for control region.

Amplicons for cox1 were Sanger sequenced using sequencing primers that were attached to species-specific primers to serve as a priming site for Sanger sequencing. Forward (ACTGCCATCTTGGAGGAC) and reverse (CCCAGGATTATGGAACGG) nondegenerate adapter tails. Sequencing was performed by Eurofins Genomics UK (Wolverhampton, UK).

Amplicons for control region were sequenced in-house using the Oxford Nanopore Technology (ONT) MinIon and the associated sequencing platform MinKnow v23.07.15. CR amplicons were used to prepare sequencing libraries with the ONT Native Barcoding Kit following the manufactures instructions. The default experimental set up was used for all sequencing runs. Basecalling and demultiplexing were performed in real time using the High accuracy (HAC) basecalling model and minimum read length set to 200 bp.

The GenBank BioProject ID for the project is PRJNA1270765.

Data collection:

Morphology laboratory

Camera: Nikon Z camera (Model N1929)

Macro lens (Nikkor Z mount MC 50mm f/2.8 or a Laowa 25mm f2.8 2.5-5x ultra).

Kaiser copy stand (RS10) with a Copy Arm RTP- 100cm.

Molecular laboratory

DNA extraction kits: PureLink(TM) Genomic DNA Mini Kit (Invitrogen, Carlsbad, USA) and DNeasy(R) Blood and Tissue Kit (Qiagen Ltd., West Sussex, UK)

Thermocycler: PCRmax Alpha Cycler version 2.41. (Antylia Scientific, AXT PTY LTD, Australia).

PCR master mix: 2x MyTaq HS master mix and (Meridian Bioscience, Bioline Reagents Ltd, UK),

ONT MinIon Mk1B (MIN-101B)

ONT Native barcoding kit 96 V14 (SQK-NBD114.96)

Metadata

File identifier

9c459656-5fe4-44f7-860f-da287111016c XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2025-05-13

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact