9d0a6437-f01e-4cf3-9f83-86d2f4ead869 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:25:26 UK GEMINI 2.3 OSGB 1936 / British National Grid Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021 2024-08-09 publication https://catalogue.ceh.ac.uk/id/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 doi: Metcalf, R., White, H.L., Moresco, V., Ormsby, M.J., Oliver, D.O., Quilliam, R. (2024). Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021. NERC EDS Environmental Information Data Centre 10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 The data includes concentrations of faecal indicator organisms isolated from the plastic waste and natural substrates found on Scottish beaches, as well as the antibiotic resistance of selected strains of these organisms. Sewage-associated plastic waste, and naturally occurring substrates (seaweed and sand), were collected in 2021 from ten beaches along the Firth of Forth estuary (Scotland, UK). The concentrations of faecal indicator organisms (FIOs), E. coli and intestinal enterococci (IE), and potential human pathogens (Vibrio spp.) on the plastic waste and natural substrates were analysed using selective media. Minimum inhibitory concentration (MIC) analysis was used to determine antibiotic resistance in selected strains. Full details about this dataset can be found at https://doi.org/10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 Metcalf, R. University of Stirling Rebecca.metcalf@stir.ac.uk https://orcid.org/0000-0002-1463-427X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author White, H.L. University of Stirling Hannah.white@stir.ac.uk author Moresco, V. University of California Riverside van.moresco@gmail.com author Ormsby, M.J. University of Stirling Michael.ormsby1@stir.ac.uk author Oliver, D.O. University of Stirling David.oliver@stir.ac.uk author Quilliam, R. University of Stirling Richard.quilliam@stir.ac.uk https://orcid.org/0000-0001-7020-4410 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Metcalf, R. University of Stirling Rebecca.metcalf@stir.ac.uk https://orcid.org/0000-0002-1463-427X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Stirling EMAIL NOT PROVIDED owner Habitats and Biotopes theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Beach pollution Environmental pathogens Plastic pollution Plastisphere Sewage discharge Wet wipes otherRestrictions Registration is required to access this data otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Metcalf, R., White, H.L., Moresco, V., Ormsby, M.J., Oliver, D.O., Quilliam, R. (2024). Concentrations and antibiotic resistance of faecal indicator organisms and human pathogens associated with sewage waste on Scottish beaches, 2021. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 textTable English utf8 environment 2021-12-01 2021-12-31 -3.878 -2.747 55.848 56.277 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/9d0a6437-f01e-4cf3-9f83-86d2f4ead869 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/9d0a6437-f01e-4cf3-9f83-86d2f4ead869.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Sewage-associated plastic waste (wipes, cotton bud sticks and sanitary products) was collected from ten beaches. All samples were stored at 4 °C and processed within 48 h. Replicate composite samples of wipes, cotton bud sticks and seaweed from each site were pre-enriched in non-selective media Luria-Bertani (LB) broth or Vibrio selective Bile Peptone broth at 37 °C in a shaking incubator for 18 h. Following pre-enrichment, serial dilutions were carried out in phosphate buffered saline (PBS) and dilutions were plated onto selective media. All plates were inverted and incubated. The presence or absence of target bacteria on all sewage-associated plastic waste samples was determined by enumerating colony forming units (CFUs). Minimum inhibitory concentration (MIC): Representative individual colonies of E. coli, IE and Vibrio spp. isolated from seaweed, wet wipe, and cotton bud stick samples, were picked off agar plates, grown further overnight in LB broth, and subjected to MIC analysis to determine antibiotic resistance to amoxicillin, ampicillin, streptomycin, cephalexin, and tetracycline at varying concentrations. Two-fold serial dilutions of each antibiotic were made in LB broth in 96-well plates. Bacterial cultures were adjusted to an optical density at a wavelength of 600nm (OD600nm) of 0.1, and 10 μL added to each well of a 96-well plate. Samples were incubated overnight at 37 °C.