9d64f2bd-2918-416f-b08c-3369edbc1b30 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:14:51 UK GEMINI 2.3 OSGB 1936 / British National Grid Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford 2025-10-20 publication https://catalogue.ceh.ac.uk/id/9d64f2bd-2918-416f-b08c-3369edbc1b30 10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30 doi: Barraclough, T.G., Sant, D.G., Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre 10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30 The data comprises both compositional data and growth assay data from an experiment of wild bacterial communities in Wytham Woods, Oxford. The communities live in puddles of water in tree-holes formed by the roots of beech trees. We manipulated the pH of tree-holes during the experiment for comparison with control tree-holes that were unmanipulated. Compositional data comprises the frequency of different Amplicon Sequencing Variants and derived measures of species diversity for samples taken over time (up to 12 weeks from the start of the experiment). The growth assay data comprises measurements of growth rates of community samples and isolates back in the laboratory, when grown on beech tea media manipulated to have different pHs. Full details about this dataset can be found at https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30 Timothy Giles Barraclough University of Oxford tim.barraclough@biology.ox.ac.uk pointOfContact Barraclough, T.G. University of Oxford tim.barraclough@biology.ox.ac.uk https://orcid.org/0000-0002-8084-2640 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Sant, D.G. Rutgers, The State University of New Jersey duhita.sant@rutgers.edu https://orcid.org/0000-0001-6865-3161 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Wong, E.L.Y. Senckenberg Biodiversity and Climate Research Centre edgar.wong@senckenberg.de author University of Oxford EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication bacterium evolution ecological community field study field experiment microbiology deciduous tree GEMET - Concepts, version 4.1.3 2019-01-28 creation Biodiversity Evolutionary ecology pH otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Barraclough, T.G., Sant, D.G., Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30 textTable English utf8 biota 2021-05-11 2021-09-29 -1.342 -1.304 51.757 51.778 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/sd/9d64f2bd-2918-416f-b08c-3369edbc1b30.zip Supporting information Supporting information available to assist in re-use of this dataset information https://data-package.ceh.ac.uk/data/9d64f2bd-2918-416f-b08c-3369edbc1b30 Download the data Download a copy of this data download dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 A field experiment was conducted in summer 2021 that surveyed bacterial communities in tree-hole puddles formed by beech tree roots at Wytham Woods, Oxford. The experiment added lime (alkali) to a set of tree-holes and compared changes in bacterial communities to control tree-holes without liming. Samples were collected before and after the manipulation, and some of these were used for growth assays in the laboratory: growing different combinations of isolates and communities on beech tea medium with modified pH.