a514a386-18d7-4925-a3d4-fb5a2922e135 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:18:54 UK GEMINI 2.3 Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves 2025-05-02 publication https://catalogue.ceh.ac.uk/id/a514a386-18d7-4925-a3d4-fb5a2922e135 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135 doi: Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135 This dataset contains experimental survival rate and transfer potential measurements of two known human pathogens, bacteria Salmonella Typhimurium and Vibrio cholerae, on plastic mulch and ready-to-eat crop plants. Colony forming units (CFUs) were used to measure persistence time of each pathogen on the mulch, and quantify the transfer of each pathogen from the plastic mulch to the surface of spinach and basil leaves. Samples were measured on days 1, 2, 3, 4, 7, 10 and 14, over a total period of 14 days. Destructive sampling methods were used to collect samples from the plastic and leaf surface, with negative control leaves also sampled at each time point. This dataset was created as part of a study investigating the risk of pathogen growth in agricultural environments using plastic mulch, and is supported by the National Environmental Research Council (NE/V005847/1). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135 Woodford, L. University of Stirling luke.woodford@stir.ac.uk pointOfContact Woodford, L. University of Stirling luke.woodford@stir.ac.uk https://orcid.org/0000-0003-2530-2120 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author White, H. University of Stirling hannah.white1@stir.ac.uk author Fellows, R. University of Stirling rosie.c.fellows@outlook.com author Ormsby, M. University of Stirling michael.ormsby@glasgow.ac.uk author Quilliam, R. University of Stirling richard.quilliam@stir.ac.uk author University of Stirling EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Human Health and Safety theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Environmental risk Pollution Agriculture plastisphere human pathogens ready-to-eat-crops phyllosphere otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135 English utf8 environment 2023-10-01 2023-11-01 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/a514a386-18d7-4925-a3d4-fb5a2922e135 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/a514a386-18d7-4925-a3d4-fb5a2922e135.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Persistence experiments, examining the survival of S. Typhimurium and V. cholerae were set up by placing spinach and basil leaves in petri dishes and adding plastic mulch with a biofilm containing each pathogen to the leaf surface. Over time samples were destructively sampled by removing the plastic, washing the leaf surface and cutting a circle from the leaf. These three sections (plastic, wash and cut leaf) were then resuspended and plated on to selevctive media. Any surviving bacteria were quantified using CFUs. Negative control leaves with no plastics or bacteria were analysed at each time point.