BGS Hosted Metadata
Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013)
This data set describes the prevalence of trypanosomes and Sodalis glossinidius, and host blood meal analysis from tsetse flies (Glossina morsitans morsitans) captured during two intensive surveys in Mambwe District, Eastern Province, Zambia in 2013. The Luangwa Valley in Zambia is an old focus of human African trypanosomiasis (HAT, Trypanosoma brucei rhodesiense) and sporadic outbreaks have continued to occur in the human population. In recent years there has been an influx of people migrating from the densely populated plateau region resulting in a significant change in land-use in the study area, potentially influencing tsetse dynamics and the epidemiology of HAT. This data set was collected to monitor infection rates of trypanosomes and Sodalis glossinidius in Glossina morsitans morsitans tsetse flies in the area so as to assess the risk posed to both human and livestock populations. In addition, feeding patterns of tsetse were investigated through analysis of blood meals. This work was part of a wider research project, the Dynamic Drivers of Disease in Africa Consortium (DDDAC) and contributed to the Zambia trypanosomiasis case study. The research was funded by NERC project no NE/J000701/1 with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345
Simple
- Date (Publication)
- 2017-03-24
- Date (Creation)
- 2016-11-21
- Citation identifier
- https://catalogue.ceh.ac.uk/id/a55eea77-8401-49ba-921e-53e085dc8345
- Citation identifier
- doi: / 10.5285/a55eea77-8401-49ba-921e-53e085dc8345
- Other citation details
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Simuunza, M., Anderson, N.E., Machila, N., Ndebe, J., Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre 10.5285/a55eea77-8401-49ba-921e-53e085dc8345
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Zambia Simuunza, M.
Point of contact The University of Edinburgh
Anderson, N.E. Point of contact University of Zambia Simuunza, M. Author The University of Edinburgh
Anderson, N.E. Author University of Zambia Machila, N. Author University of Zambia Ndebe, J. Author The University of Edinburgh
Macleod, E.T.
Author The University of Edinburgh
Welburn, S.C. Author NERC EDS Environmental Information Data Centre Custodian NERC Environmental Information Data Centre Publisher
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Wikidata
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GeoNames
- Keywords
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Luangwa Valley
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Dynamic Drivers of Disease in Africa Consortium (DDDAC)
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
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- Other restrictions
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© The University of Edinburgh
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© University of Zambia
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If you reuse this data, you should cite: Simuunza, M., Anderson, N.E., Machila, N., Ndebe, J., Macleod, E.T., Welburn, S.C. (2017). Molecular analysis of Glossina morsitans morsitans tsetse from Mambwe District, Zambia (2013). NERC Environmental Information Data Centre https://doi.org/10.5285/a55eea77-8401-49ba-921e-53e085dc8345
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Health
- Begin date
- 2013-05-01
- End date
- 2013-11-30
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/a55eea77-8401-49ba-921e-53e085dc8345 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/a55eea77-8401-49ba-921e-53e085dc8345.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Tsetse surveys were conducted in May / June 2013 and November 2013. Tsetse flies were sampled using black screen fly rounds. Data were recorded on standard field sampling forms and then manually transferred to Microsoft Excel. Data were then checked for accuracy and incorrect spatial location coordinates removed. Tsetse flies were homogenized and DNA extracted. The resulting DNA was stored at minus 20 degrees Celsius until needed for Polymerase chain reaction (PCR). PCR analysis was performed using different primers to detect the different species of trypanosomes in the DNA. All PCR included a negative and a positive control. To determine the sources of blood meal (feeding patterns), tsetse DNA samples were subjected to PCR targeting the cytochrome oxidase b gene. The resulting PCR product was sequenced, and the sequences compared with those available in public databases by using nucleotide BLAST at NCBI website ( http://blast.ncbi.nlm.nih.gov/Blast.cgi) to determine the animal species on which tsetse fed on. Data were collated in a Microsoft Excel spreadsheet and exported as a comma separated file for ingestion into the Environmental Information Data Centre (EIDC).
Metadata
- File identifier
- a55eea77-8401-49ba-921e-53e085dc8345 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:26:12
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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