abbc69c2-b799-4892-a339-fb1ffa4ea2f0 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:16:04 UK GEMINI 2.3 Colonisation rates of E. coli on plastic and banana leaf under simulated environmental conditions 2025-06-12 publication https://catalogue.ceh.ac.uk/id/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 10.5285/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 doi: Ormsby, M., Woodford, L., Fellows, R., White, H., Quilliam, R. (2025). Colonisation rates of E. coli on plastic and banana leaf under simulated environmental conditions. NERC EDS Environmental Information Data Centre 10.5285/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 This dataset contains information relating to the rate of colonisation of E. coli O157 on plastic and banana leaf, under simulated environmental conditions. The data encompass bacterial strain identity, material type, timepoints (days), temperature (°C), and bacterial concentration (CFU/ml). Data were sampled over defined periods using culture-based approaches. This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1]. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 Michael Ormsby University of Stirling Michael.Ormsby@glasgow.ac.uk pointOfContact Ormsby, M. University of Stirling michael.ormsby@glasgow.ac.uk https://orcid.org/0000-0002-3991-2336 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Woodford, L. University of Stirling Luke.woodford@stir.ac.uk https://orcid.org/0000-0003-2530-2120 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Fellows, R. University of Stirling rosie.c.fellows@outlook.com https://orcid.org/0009-0003-8928-2189 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author White, H. University of Stirling Hl-white@outlook.com https://orcid.org/0000-0001-9842-7798 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Quilliam, R. University of Stirling Richard.quilliam@stir.ac.uk https://orcid.org/0000-0001-7020-4410 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Stirling EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher Environmental risk Water quality Plastisphere Biofilm Wastewater E. coli O157 AMR otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Ormsby, M., Woodford, L., Fellows, R., White, H., Quilliam, R. (2025). Colonisation rates of E. coli on plastic and banana leaf under simulated environmental conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 English utf8 environment Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/abbc69c2-b799-4892-a339-fb1ffa4ea2f0 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/abbc69c2-b799-4892-a339-fb1ffa4ea2f0.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Data were generated through laboratory experiments investigating the persistence, and survival of a clinically relevant strain of E. coli. Bacteria were cultured on materials (Banana Leaf and HDPE) and analysed for persistence over time. Materials (Banana Leaf and HDPE) were prepared and inoculated under controlled laboratory conditions. E. coli strains were inoculated onto materials in nutrient-rich and surface water environments. Sampling was taken at defined time points (7, 14, 21, 28 days). The experiments utilised standard laboratory equipment such as 96-well plates and incubators. Instrumentation used for bacterial enumeration, including the plate reader, was calibrated according to standard laboratory protocols. The data underwent rigorous quality control through replication (biological triplicates), statistical analysis (ANOVA), and controlled experimental conditions (e.g., temperature and material consistency). Statistical methods including ANOVA and Tukey’s post-hoc tests were applied to assess treatment effects and survival outcomes.