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&#xD;
The Southern Ocean Clouds was supported by NERC as part of the CloudSense Programme and it was funded by the project grant number NE/T006404/1.</gco:CharacterString>
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At East Beach hut, in Rothera, 30 Nuclepore Hydrophilic Membrane filters (see details below) were loaded in to filter holders for the magazine of the DIGITEL DPA14 LoVol automatic sampler. To avoid contamination, the filter holders and materials were cleaned with IPA and lint free wipes and the work was conducted inside a laminar flow hood. The DIGITEL DPA14 was set to change filters every 48 hours, and to sample at a flow rate of 15 Litres per minute. For each batch of 30 filters, at least 1 filter was not exposed to air and used as a handling blank to assess background contamination. Once the DIGITEL DPA14 had run through all of the filters, the magazine and filter holders were emptied in the laminar flow hood and each filter was packed in a separate, numbered plastic bag. The filters were stored and shipped to the British Antarctic Survey in Cambridge at -20C.&#xD;
&#xD;
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Data collection: 
Sample collection: DIGITEL DPA14 automatic filter sampler, Nuclepore Hydrophilic Membrane, 0.2 &amp;#956;m pore size, 47 mm track edged filters (10417012).&#xD;
Sample analysis: For methodology see: Budke and Koop, 2015. &#xD;
cooling: Linkam LTS120 with T95-LinkPad temperature controlled stage, Julabo F250 recirculating cooler. &#xD;
set-up: Marienfeld (MARI0895040) hydrophobic glass slides, Sigma-Aldrich (S3626-100ML) squalene. Microsoft LifeCam webcam. In-house built droplets spacer, holder and lighting&#xD;
Software: (picture taking and processing) in-house Python software
Data quality: 
Each batch of 30 automatically collected filters from the DIGITEL DPA14 contained at least one filter which had not been exposed to ambient air and was therefore kept as a handling blank. The median temperature for the handling blanks was -26.8C and we therefore consider -26C as a reasonable cut-off above which we can distinguish ice nucleating activity on our filters. &#xD;
&#xD;
We also ran Milli-Q blanks on each day before analysing filter samples to assess any background contamination in the lab or in the Milli-Q water system. We would not run any samples if the temperatures for this blank were above the handling blank temperature of -26C.&#xD;
&#xD;
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