b0aec477-0883-4963-a3ae-52e3f0daf5aa English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-01-09T10:20:31 UK GEMINI 2.3 Mimetic host shifts in a social parasite of ants: Analytical data of surface semio-chemicals on Maculinea rebeli larvae 2013-01-22 publication 1351093101460 CEH:EIDC: https://catalogue.ceh.ac.uk/id/b0aec477-0883-4963-a3ae-52e3f0daf5aa 10.5285/b0aec477-0883-4963-a3ae-52e3f0daf5aa doi: Schönrogge, K., Thomas, J.A., Elmes, G.W., Sielezniew, M., Stankiewicz-Fiedurek, A., Simcox, D., Settele, J. (2013). Mimetic host shifts in a social parasite of ants: Analytical data of surface semio-chemicals on Maculinea rebeli larvae. NERC Environmental Information Data Centre 10.5285/b0aec477-0883-4963-a3ae-52e3f0daf5aa This dataset is part of the study of mimetic host shifts in an endangered social parasite of ants, which is a joint study of the Centre for Ecology & Hydrology and the University of Oxford. It contains the relative abundance data of cuticular hydrocarbons extracted from worker ants of Myrmica sabuleti and M. schencki, and from caterpillars of Maculinea rebeli from two populations at the pre-adoption stage and after being reared by the two ant species. The chemical was analysed from the caterpillars from each region when reared with each ants, by using gas chromatography with mass spectrometric detection (GC-MSD). Various statistical analysis was then carried out to compare the differences between groups. It aims to test whether observed regional differences in M. rebeli's host specificity could be explained by variation in chemical mimicry. Detailed research method can be found in Thomas et al. (2013) Mimetic host shifts in an endangered social parasite of ants. Proc. R. Soc. B vol. 280 no.1751. (https://doi.org/10.1098/rspb.2012.2336) Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/b0aec477-0883-4963-a3ae-52e3f0daf5aa Schönrogge, K. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Schönrogge, K. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0003-0122-6493 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Thomas, J.A. University of Oxford enquiries@ceh.ac.uk author Elmes, G.W. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Sielezniew, M. University of Bialystok enquiries@ceh.ac.uk author Stankiewicz-Fiedurek, A. Polish Academy of Sciences enquiries@ceh.ac.uk author Simcox, D. Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Settele, J. UFZ Helmholtz Centre for Environmental Research enquiries@ceh.ac.uk author NERC Environmental Information Data Centre info@eidc.ac.uk publisher notPlanned Myrmica schencki Myrmica sabuleti Wikidata 2012-10-29 creation Animal behaviour Maculinea rebeli otherRestrictions Registration is required to access this data otherRestrictions Licence terms and conditions apply otherRestrictions You must acknowledge the following resource: Thomas et al. (2013). Mimetic host shifts in an endangered social parasite of ants. Proc. R. Soc. B vol. 280 no.1751. (doi:10.1098/rspb.2012.2336) otherRestrictions If you reuse this data, you should cite: Schönrogge, K., Thomas, J.A., Elmes, G.W., Sielezniew, M., Stankiewicz-Fiedurek, A., Simcox, D., Settele, J. (2013). Mimetic host shifts in a social parasite of ants: Analytical data of surface semio-chemicals on Maculinea rebeli larvae. NERC Environmental Information Data Centre https://doi.org/10.5285/b0aec477-0883-4963-a3ae-52e3f0daf5aa English utf8 biota 2001-01-01 2005-12-31 Comma-separated values (CSV) Environmental Information Data Centre Hub info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/b0aec477-0883-4963-a3ae-52e3f0daf5aa Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/b0aec477-0883-4963-a3ae-52e3f0daf5aa.zip Documents Documents available to assist with re-use of this dataset. information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 We tracked the changing chemical profiles of caterpillars from each region when reared with each ant, from uncontaminated pre-adoption final instars, to individuals 6 weeks after adoption, and finally to the latter after isolation from ants for 5 days, which allows acquired semio-chemicals to dissipate and prompts the hungry caterpillars to release their own secretions. Hexane extracts of surface chemicals were obtained from: (i) unparasitized Myrmica schencki and M. sabuleti on our study sites in the Pyrenees, Poland, and the naïve ants in France that had not experienced M. rebeli (n = 5 workers from 5 nests of each ant per locality); (ii) 8 batches per region of pre-adoption final instar M. rebeli larvae sampled after leaving G. cruciata before contact with ants (n = 5 larvae per batch, in total 40 individuals for each type of M. rebeli); (iii) M. rebeli larvae after living 6 weeks with naïve French ants, n = 5 (Spanish+schencki and Polish+sabuleti), n = 3 (Spanish+sabuleti and Polish+schencki); and (iv) M. rebeli larvae reared as in (iii), then isolated from ants and kept unfed and singly in sterile conditions for 5 days; n = 5 (Spanish+schencki), n = 4 (Polish+schencki), n = 3 (Spanish+sabuleti and Polish+sabuleti). The chemical and statistical analyses of extracts followed an established protocol. Maculinea extracts were concentrated to 20ml, ant workers to 50ml, and 2ml of every sample were analyzed by gas chromatography with mass spectrometric detection (GC-MSD) using a HP 5890II GC and HP 5971A MSD, and ultra-high purity helium as the carrier gas with 10 psi column head pressure. Mass spectral data were acquired in full scan mode over 40 - 600 m/z. Mass chromatograms were initially screened for hydrocarbons by examining the selected ion chromatogram of m/z = 57. The chromatogram was integrated at a threshold value of 12 (HP integrator) to obtain the areas under the peaks measuring the total ion count. With each sequence of samples we also analysed alkane standards (n-C20 - n-C36), and the position of each peak within that range in a sample was calculated as an Equivalent Chain Length (ECL). Mass chromatograms were inspected to ensure that they were free of gross interferences and that peaks of interest such as branched and straight alkanes and alkenes, were chromatographically distinct and symmetrical. We excluded peaks that were column bleed, siloxanes, or phthalate plasticizers as indicated by a characteristic abundant ion at m/z 149. Peaks of interest were tentatively identified by a combination of ECL number and inspections of their full scan mass spectra and matching with the NIST-97/08 mass spectral database. For statistical analysis, the area under each peak was expressed as the proportion of the sum of all peaks in the chromatogram. Samples were compared using multivariate and non-parametric multidimensional scaling (MDS) on the ranks of the Bray-Curtis similarities. The extent of a final lack of fit was assessed by a STRESS statistic before pair-wise differences between species and treatments were assessed using an analysis of similarities (ANOSIM) in Primer-e v6. We used the average pairwise distance between groups, and assessed two averages with a two-sample t-test, to compare differences in the shift of similarities between groups.